Yarat A, Ozçelik F, Emekli N
Department of Biochemistry, Faculty of Dentistry, Marmara University, Istanbul, Türkiye.
J Marmara Univ Dent Fac. 1996 Sep;2(2-3):527-9.
The aim of this research was to develop a method of local production of collagen graft materials which are presently imported. The following methods were used to produce collagen membrane and sponge from human placentas and rat tail tendons. Collagen type I was isolated from human placenta and rat tail tendon by acetic acid extraction and characterised by SDS-PAGE. The collagen sponge was prepared by dissolving the collagen in HCl. The resulting dispersion was poured into a glass container, freeze-dried and then cross-linked by immersion in glutaraldehyde solution. It was then washed with distilled water and freeze-dried again. The collagen membrane was also similarly prepared by dispersing lyophilized collagen in HCl but then mixed with glutaraldehyde, exposed to U.V. light and later air dried.
本研究的目的是开发一种目前依赖进口的胶原蛋白移植材料的本地生产方法。采用以下方法从人胎盘和大鼠尾腱制备胶原蛋白膜和海绵。通过乙酸萃取从人胎盘和大鼠尾腱中分离出I型胶原蛋白，并通过SDS-PAGE进行表征。将胶原蛋白溶解在盐酸中制备胶原蛋白海绵。将所得分散液倒入玻璃容器中，冷冻干燥，然后浸入戊二醛溶液中进行交联。然后用蒸馏水洗涤并再次冷冻干燥。胶原蛋白膜的制备方法类似，即将冻干的胶原蛋白分散在盐酸中，但随后与戊二醛混合，暴露于紫外线下，然后风干。
