Atsumi T, Murata J, Kamiyanagi I, Fujisawa S, Ueha T
Department of Physiology, Meikai University, School of Dentistry, Saitama, Japan.
Arch Oral Biol. 1998 Jan;43(1):73-81. doi: 10.1016/s0003-9969(97)00073-3.
The cytotoxic effect of two types of photosensitizers (camphorquinone, CQ, a widely used aliphatic type and 9-fluorenone, 9F, an aromatic type) in the presence of 2-dimethylaminoethyl methacrylate (DM) as a reducing agent with exposure to visible light (350-550 nm) was examined in a human cell line. Cytotoxicity was evaluated in terms of the percentage of cell survival, and the production of reactive oxygen in living single cells was measured with an adherent cell analysis and sorting laser cytometer and a peroxide indicator. The amount of reactive oxygen generated in the cells irradiated in the 9F (1 mM-3 min) system was about 9-fold greater than under the same conditions in the CQ system. Similarly, the decrease in cell survival in the 9F system was about 10-fold greater than in the CQ. Both the production of reactive oxygen in the cells and the decrease in cell survival paralleled the concentration of photosensitizers and the irradiation time. Although the cell-damaging effects with the CQ system were mild, at a higher dose (10 mM) and longer irradiation time (24 min) it produced cell survival equal to that in the 9F (1 mM-3 min) system. These results suggest that in the case of irradiated photosensitizer systems, 9F was much more damaging to the cells than CQ, which damage probably occurred via free radicals involving reactive oxygen generation.
在人细胞系中，研究了两种光敏剂（樟脑醌，CQ，一种广泛使用的脂肪族型光敏剂；以及9-芴酮，9F，一种芳香族型光敏剂）在作为还原剂的甲基丙烯酸2-二甲氨基乙酯（DM）存在下，经可见光（350 - 550 nm）照射后的细胞毒性作用。根据细胞存活百分比评估细胞毒性，并用贴壁细胞分析和分选激光细胞仪以及过氧化物指示剂测量活单细胞中的活性氧生成量。在9F（1 mM - 3分钟）体系中照射的细胞所产生的活性氧量比相同条件下CQ体系中的约高9倍。同样，9F体系中细胞存活率的降低比CQ体系中的约高10倍。细胞中活性氧的产生以及细胞存活率的降低均与光敏剂浓度和照射时间平行。尽管CQ体系的细胞损伤作用较轻，但在较高剂量（10 mM）和较长照射时间（24分钟）时，其导致的细胞存活率与9F（1 mM - 3分钟）体系中的相当。这些结果表明，在照射的光敏剂体系中，9F对细胞的损伤比CQ大得多，这种损伤可能是通过涉及活性氧生成的自由基发生的。
