Ozer E, Canda T, Kurtodlu B
Department of Pathology, Dokuz Eylul University Hospital, Inciralti, Izmir, Turkey.
Cancer Lett. 1997 Dec 23;121(2):119-23. doi: 10.1016/s0304-3835(97)00346-7.
To investigate the role of tumor angiogenesis, laminin and CD44 expression in metastatic potential of breast cancers, 35 early-stage (T1) and/or low-grade (grade 1) invasive breast carcinomas including 18 metastasizing and 17 non-metastasizing cases were analyzed in the present study. Angiogenesis was assessed by a stereologic method after immunohistochemical staining of vascular endothelium for factor VIII. The quantification of the tumor vascularization was based on the vascular surface density (VSD) and the microvessel number (NVES). The expressions of laminin and CD44 were evaluated by scoring the intensity and distribution of the immunostaining. The assessment of NVES and VSD resulted in a statistically significant difference between the two groups (P < 0.05, independent-samples t-test). In the breast cancers with metastatic spread, NVES was found to be 16.0+/-2.9 mm(-2) whereas it was 8.1+/-0.7 mm(-2) in the metastasizing group. VSD was found to be 59.0+/-12.6 mm(-1) in the metastasizing group and was significantly lower in the non-metastasizing group (33.8+/-2.6 mm[-1]). Immunohistochemistry exhibited strongly positive staining for CD44 in 22% of the breast cancers with metastasis, while 65% of the non-metastasizing cases were found to be negative. The statistical analysis also resulted in a significant difference (P = 0.034, chi2 test). However, the immunohistochemical staining for laminin did not yield any significant difference (P = 0.347, chi2 test). Approximately half of the cases being either metastasizing or non-metastasizing stained weakly positive for laminin. We concluded that angiogenesis and CD44 expression in breast cancers correlated significantly with lymph node or distant metastasis. However, the immunodetection of laminin expression in our study did not help to evaluate its role in the metastatic potential of breast cancers. We concluded that vascular parameters, such as an increase in microvessel number, and CD44 expression might be useful prognostic indicators of metastasis in breast cancer.
为研究肿瘤血管生成、层粘连蛋白和CD44表达在乳腺癌转移潜能中的作用，本研究分析了35例早期（T1）和/或低级别（1级）浸润性乳腺癌，其中包括18例发生转移的病例和17例未发生转移的病例。血管生成通过对血管内皮因子VIII进行免疫组织化学染色后采用体视学方法进行评估。肿瘤血管化的量化基于血管表面密度（VSD）和微血管数量（NVES）。层粘连蛋白和CD44的表达通过对免疫染色的强度和分布进行评分来评估。NVES和VSD的评估结果显示两组之间存在统计学显著差异（P<0.05，独立样本t检验）。在发生转移的乳腺癌中，NVES为16.0±2.9mm(-2)，而在未发生转移的组中为8.1±0.7mm(-2)。转移组的VSD为59.0±12.6mm(-1)，在未发生转移的组中显著更低（33.8±2.6mm[-1]）。免疫组织化学显示，22%发生转移的乳腺癌中CD44染色呈强阳性，而65%未发生转移的病例为阴性。统计分析也显示存在显著差异（P = 0.034，卡方检验）。然而，层粘连蛋白的免疫组织化学染色未显示任何显著差异（P = 0.347，卡方检验）。大约一半发生转移或未发生转移的病例层粘连蛋白染色呈弱阳性。我们得出结论，乳腺癌中的血管生成和CD44表达与淋巴结或远处转移显著相关。然而，我们研究中层粘连蛋白表达的免疫检测无助于评估其在乳腺癌转移潜能中的作用。我们得出结论，血管参数，如微血管数量增加，以及CD44表达可能是乳腺癌转移的有用预后指标。
