Yatsunami J, Tsuruta N, Ogata K, Wakamatsu K, Takayama K, Kawasaki M, Nakanishi Y, Hara N, Hayashi S
Research Institute for Disease of the Chest, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer Lett. 1997 Nov 25;120(1):101-8. doi: 10.1016/s0304-3835(97)00296-6.
We examined interleukin-8 (IL-8) production in 17 lung cancer cell lines, IL-8 expression in tumor specimens and IL-8's contribution to tumor-induced angiogenesis in vivo. Eight of 13 non-small cell lung cancer cell lines constitutively produced high levels of IL-8. Four small cell lung cancer cell lines produced little or no IL-8. Immunohistochemical analysis of transbronchial biopsy specimens revealed IL-8 staining within adenocarcinomas (22/32), squamous cell carcinomas (12/21) and large cell carcinomas (2/3), but not within most small cell carcinomas (1/22). Anti-IL-8 antisera blocked tumor angiogenesis by two IL-8 producing cell lines in a mouse model.
我们检测了17种肺癌细胞系中白细胞介素-8（IL-8）的产生、肿瘤标本中IL-8的表达以及IL-8在体内对肿瘤诱导血管生成的作用。13种非小细胞肺癌细胞系中有8种组成性地产生高水平的IL-8。4种小细胞肺癌细胞系产生很少或不产生IL-8。经支气管活检标本的免疫组织化学分析显示，腺癌（22/32）、鳞状细胞癌（12/21）和大细胞癌（2/3）中有IL-8染色，但大多数小细胞癌（1/22）中没有。在小鼠模型中，抗IL-8抗血清可阻断两种产生IL-8的细胞系的肿瘤血管生成。
