Chen Xiaojia, Lu Jia, Ji Yuhua, Hong An, Xie Qiuling
Guangdong Provincial Key Laboratory of Bioengineering Medicine, Guangzhou, 510632, China,
Tumour Biol. 2014 Jan;35(1):791-8. doi: 10.1007/s13277-013-1109-0. Epub 2013 Sep 12.
Bone is one of the most common metastatic sites for breast cancer. In this study, we observed a promoting effect of osteoblast-conditioned medium (OCM) on the migration of MCF-7, a noninvasive cell line of breast cancer cells. Cytokine antibody array was used to compare the cytokines of OCM with the conditioned medium of non-differentiated osteoblast cells, which consequently revealed factors related to migration, such as IL8, IL6, CSF2 (G-CSF), CSF3 (GM-CSF), and TNFRSF11B (osteoprotegerin). The expression of genes related to migration was also estimated with a PCR array, which showed that 9 genes were upregulated and 26 genes downregulated. Moreover, activated p38, ERK, and AKT pathways were found in the OCM treatment group. This finding indicated the migration ability of breast cancer cells, which move toward the bone depending on the presence of specific cytokines in its surrounding microenvironment.
骨是乳腺癌最常见的转移部位之一。在本研究中，我们观察到成骨细胞条件培养基（OCM）对MCF-7（一种乳腺癌非侵袭性细胞系）迁移的促进作用。使用细胞因子抗体阵列比较OCM与未分化成骨细胞条件培养基中的细胞因子，从而揭示了与迁移相关的因子，如IL8、IL6、CSF2（G-CSF）、CSF3（GM-CSF）和TNFRSF11B（骨保护素）。还通过PCR阵列评估了与迁移相关基因的表达，结果显示9个基因上调，26个基因下调。此外，在OCM处理组中发现p38、ERK和AKT通路被激活。这一发现表明乳腺癌细胞具有迁移能力，其会根据周围微环境中特定细胞因子的存在向骨迁移。
