Reader T A, Strazielle C, Botez M I, Lalonde R
Centre de Recherche en Sciences Neurologiques, Faculté de Médecine, Université de Montréal, Québec, Canada.
Brain Res Bull. 1998 Mar 15;45(5):489-93. doi: 10.1016/s0361-9230(97)00430-9.
Lurcher mutant mice are characterized by massive degeneration of the cerebellum, including Purkinje cells and granule cells, as well as for the loss of neurons from the inferior olive. Concentrations of dopamine and two of its metabolites and of several amino acid neurotransmitters were determined in the cerebellum and in other brain regions of these mutants. By comparison to wild-type mice of the same background strain, glutamate and taurine concentrations were reduced in the Lurcher cerebellum. No decrease was found for aspartate, gamma-aminobutyric acid (GABA), glycine, as well as dopamine and its metabolites. Moreover, no neurochemical alterations occurred in the brain stem, thalamus, or neostriatum of Lurcher mutants. A selective reduction of glutamate concentration was found in the hippocampus, while all amino acids measured were decreased in the entorhinal-piriform areas. These results indicate region-selective reductions of neurotransmitter concentrations in a mouse mutant with a defined cerebellar cortical pathology.
蹒跚突变小鼠的特征是小脑出现大量退化，包括浦肯野细胞和颗粒细胞，以及下橄榄核神经元的丧失。测定了这些突变小鼠小脑和其他脑区中多巴胺及其两种代谢产物以及几种氨基酸神经递质的浓度。与相同背景品系的野生型小鼠相比，蹒跚小鼠小脑中谷氨酸和牛磺酸的浓度降低。天冬氨酸、γ-氨基丁酸（GABA）、甘氨酸以及多巴胺及其代谢产物未发现降低。此外，蹒跚突变小鼠的脑干、丘脑或新纹状体未发生神经化学改变。海马体中发现谷氨酸浓度选择性降低，而在内嗅梨状区，所有测定的氨基酸浓度均降低。这些结果表明，在具有明确小脑皮质病理的小鼠突变体中，神经递质浓度存在区域选择性降低。
