Reader T A, Le Marec N, Ase A R, Lalonde R
Centre de Recherche en Sciences Neurologiques, Département de physiologie, Faculté de médecine, Université de Montréal, Québec, Canada.
Neurochem Res. 1999 Sep;24(9):1125-34. doi: 10.1023/a:1020708319483.
The biochemical parameters of the serotoninergic system were examined in wild type mice and Lurcher mutants after chronic treatment (40 days) with the serotonin (5-HT) precursor L-tryptophan (50 mg/kg; i.p.). Tissue contents in 5-HT, dopamine and noradrenaline, as well as some of their metabolites, were measured in frontal cortex, neostriatum, thalamus, brainstem, cerebellum and spinal cord by high-performance liquid chromatography. The tissue levels were used as a biochemical index of the function of the monoamine innervations in this animal model of cerebellar ataxia. The results show that Lurcher mutants retain higher concentrations of L-tryptophan and total indoleamines, but that 5-HT is probably stored in a non-releasable compartment. In the particular case of the hypoplastic cerebellum, the reorganization of 5-HT nerve terminals leads to an accrued indoleamine synthesis, indicating that the Lurcher mutants can accumulate 5-HT, but do not utilize it efficiently in synaptic transmission.
在用血清素（5-羟色胺，5-HT）前体L-色氨酸（50毫克/千克；腹腔注射）对野生型小鼠和Lurcher突变体进行慢性治疗（40天）后，检测了血清素能系统的生化参数。通过高效液相色谱法测量额叶皮质、新纹状体、丘脑、脑干、小脑和脊髓中5-HT、多巴胺和去甲肾上腺素及其一些代谢产物的组织含量。这些组织水平被用作这个小脑共济失调动物模型中单胺神经支配功能的生化指标。结果表明，Lurcher突变体中L-色氨酸和总吲哚胺的浓度较高，但5-HT可能储存在一个不可释放的隔室中。在发育不全的小脑这种特殊情况下，5-HT神经末梢的重组导致吲哚胺合成增加，这表明Lurcher突变体可以积累5-HT，但在突触传递中不能有效地利用它。
