Sasagawa I, Tateno T, Adachi Y, Kubota Y, Nakada T
Department of Urology, Yamagata University School of Medicine, Japan.
J Androl. 1998 Mar-Apr;19(2):196-200.
We investigated fertilization and embryo development after intracytoplasmic injection of round spermatids collected from mice before puberty. The rates of normal fertilization, blastocyst formation, and development into live offspring were examined. The injection of oocytes with round spermatids from mature or prepubertal males resulted in similar rates of normal fertilization and embryo development. The rates of the development to term of two-cell embryos were also similar, regardless of the origin of the round spermatids injected. This finding suggests that round spermatids from the prepubertal testis already have the ability to fertilize normally and to allow normal embryo development.
我们研究了从青春期前小鼠采集的圆形精子细胞经胞浆内注射后的受精和胚胎发育情况。检测了正常受精率、囊胚形成率以及发育为活仔的情况。用来自成熟或青春期前雄性小鼠的圆形精子细胞注射卵母细胞，其正常受精率和胚胎发育率相似。无论注射的圆形精子细胞来源如何，两细胞胚胎发育至足月的比率也相似。这一发现表明，青春期前睾丸中的圆形精子细胞已经具备正常受精和支持正常胚胎发育的能力。
