注射了冷冻保存的圆形精子细胞的小鼠卵母细胞能够发育成正常后代。
Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring.
作者信息
Ogura A, Matsuda J, Asano T, Suzuki O, Yanagimachi R
机构信息
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
出版信息
J Assist Reprod Genet. 1996 May;13(5):431-4. doi: 10.1007/BF02066177.
PURPOSE
This study was performed to determine whether frozen-thawed mouse round spermatids can fertilize oocytes and contribute to normal embryo development.
METHODS
Freshly collected mouse testicular cells were frozen in PBS containing 7.5% glycerol and 7.5% fetal bovine serum. After thawing and removal of the cryoprotectants, round spermatids were selected and injected individually into mature oocytes which had been previously activated with Sr(2+)-containing Ca(2+)-free medium.
RESULTS
After thawing, 75-85% of testicular cells were alive. About 90% of the oocytes were fertilized by intracytoplasmic injection of frozen-thawed round spermatids; 11% (17/150) of embryos transferred to foster mothers developed into normal offspring.
CONCLUSIONS
Mouse round spermatids can be cryopreserved for production of normal offspring.
目的
进行本研究以确定冻融后的小鼠圆形精子细胞是否能够使卵母细胞受精并促进正常胚胎发育。
方法
将新鲜采集的小鼠睾丸细胞冻存于含有7.5%甘油和7.5%胎牛血清的PBS中。解冻并去除冷冻保护剂后，挑选出圆形精子细胞，将其逐个注射到先前已用含Sr(2+)的无Ca(2+)培养基激活的成熟卵母细胞中。
结果
解冻后，75 - 85%的睾丸细胞存活。通过胞质内注射冻融后的圆形精子细胞，约90%的卵母细胞受精；移植到代孕母鼠体内的胚胎中有11%（17/150）发育为正常后代。
结论
小鼠圆形精子细胞可进行冷冻保存以产生正常后代。
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