Boyd Y
MRC Mammalian Genetics Unit, Harwell, Oxon, United Kingdom.
Methods. 1998 Feb;14(2):120-34. doi: 10.1006/meth.1997.0572.
This article provides an introduction to genetic mapping for scientists who wish to map specific genes or mutant phenotypes in the mouse. The basic principles of genetic mapping and the different types of genetic markers available are described in the first two sections of the chapter. The theoretical and empirical principles necessary to consider when designing mapping experiments are reviewed in the third section. Protocols for mapping phenotypic traits and cloned genes are detailed in the fourth and fifth sections.
本文为希望在小鼠中定位特定基因或突变表型的科学家提供了基因定位的介绍。本章的前两节描述了基因定位的基本原理以及可用的不同类型的遗传标记。第三节回顾了设计定位实验时需要考虑的理论和经验原理。第四节和第五节详细介绍了定位表型性状和克隆基因的实验方案。
