在结节病患者中，脂皮质素I基因在血液单核细胞中的表达高于支气管肺泡灌洗（BAL）液巨噬细胞中的表达。

Lipocortin I gene expression is higher in blood monocytes than in BAL fluid macrophages in patients with pulmonary sarcoidosis.

作者信息

Mikuniya T, Nagai S, Morita K, Mio T, Satake N, Izumi T

机构信息

Chest Disease Research Institute, Kyoto University, Japan.

出版信息

Sarcoidosis Vasc Diffuse Lung Dis. 1998 Mar;15(1):38-43.

PMID:9572000

Abstract

BACKGROUND AND AIM OF WORK

Lipocortin I, induced by corticosteroids in vivo is considered to be related to cell activation and differentiation in mononuclear phagocytes, key cells in the process of epithelioid cell granuloma formation in sarcoidosis. Therefore, we focused on the expression of lipocortin I gene in mononuclear phagocytes in patients with sarcoidosis.

METHOD

The amounts of transcripts of lipocortin I gene obtained from both BAL fluid macrophages and blood monocytes were measured by RT-PCR and HPLC, and then standardized as the ratio of lipocortin I/beta-actin for comparison.

RESULTS

Smoking did not affect the expression of lipocortin I gene in BAL fluid macrophages and monocytes. 2. There were no differences in the amounts of expression in BAL fluid macrophages between patient groups (nonsmokers and smokers), or between patients and healthy subjects. 3. The gene expression in BAL fluid macrophages was higher than that in monocytes in patients with IPF (macrophages 1.23 +/- 0.48, vs monocytes 0.50 +/- 0.38, p < 0.05) and healthy subjects (nonsmokers: 1.66 +/- 1.17, vs 0.34 +/- 0.26, p < 0.05, smoker: 1.98 +/- 0.96, vs 0.34 +/- 0.48, p < 0.05, but sarcoidosis patients showed a higher expression of lipocortin I gene even in monocytes (2.62 +/- 1.87, vs 2.66 +/- 2.21).

CONCLUSION

The increase in lipocortin I gene expression in monocytes was a disease-related finding in sarcoidosis.

摘要

工作背景与目的

体内皮质类固醇诱导产生的脂皮质蛋白I被认为与单核吞噬细胞的细胞活化和分化有关，单核吞噬细胞是结节病上皮样细胞肉芽肿形成过程中的关键细胞。因此，我们重点研究了结节病患者单核吞噬细胞中脂皮质蛋白I基因的表达情况。

方法

通过逆转录聚合酶链反应（RT-PCR）和高效液相色谱法（HPLC）测定从支气管肺泡灌洗（BAL）液巨噬细胞和血液单核细胞中获取的脂皮质蛋白I基因转录本的量，然后将其标准化为脂皮质蛋白I/β-肌动蛋白的比值进行比较。

结果

吸烟不影响BAL液巨噬细胞和单核细胞中脂皮质蛋白I基因的表达。2. 患者组（非吸烟者和吸烟者）之间或患者与健康受试者之间，BAL液巨噬细胞中的表达量没有差异。3. 特发性肺纤维化（IPF）患者和健康受试者中，BAL液巨噬细胞中的基因表达高于单核细胞（IPF患者：巨噬细胞1.23±0.48，单核细胞0.50±0.38，p<0.05；健康非吸烟者：1.66±1.17，单核细胞0.34±0.26，p<0.05；健康吸烟者：1.98±0.96，单核细胞0.34±0.48，p<0.05），但结节病患者即使在单核细胞中脂皮质蛋白I基因的表达也较高（2.62±1.87，单核细胞2.66±2.21）。