Miles M P, Leach S K, Kraemer W J, Dohi K, Bush J A, Mastro A M
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
J Appl Physiol (1985). 1998 May;84(5):1604-9. doi: 10.1152/jappl.1998.84.5.1604.
We hypothesized that expression of L-selectin and very late antigen-4 (VLA-4) integrin adhesion molecules would influence cell type-specific redistribution during exercise. Women subjects performed six sets of 10-repetition maximum squats. L-selectin and VLA-4 integrin were measured by using flow cytometry pre- and postexercise on peripheral blood neutrophils and lymphocytes (n = 29 subjects) and lymphocyte subsets (n = 70 subjects), respectively. Neutrophil concentration increased 41.8% (P < 0.001), whereas the percent expressing L-selectin was unchanged (79%). Lymphocyte concentration increased 61.8% (P < 0.001). The percent of T cells expressing L-selectin decreased from 73.5 +/- 8.9 to 68.2 +/- 11.4% (P < 0.001); the combined population of natural killer and B cells expressing L-selectin decreased from 80.4 +/- 22.5 to 62.7 +/- 25.8% (P < 0.001). VLA-4 integrin was expressed by nearly all lymphocytes both pre- and postexercise. The proportional decrease in L-selectin positive cells could have resulted from 1) shedding of L-selectin, 2) selective entry of L-selectin-negative subsets, or 3) selective removal of L-selectin-positive subsets.
我们假设L-选择素和极晚期抗原-4（VLA-4）整合素粘附分子的表达会影响运动过程中细胞类型特异性的重新分布。女性受试者进行了六组每组10次的最大深蹲练习。分别在运动前和运动后，通过流式细胞术对外周血中性粒细胞和淋巴细胞（n = 29名受试者）以及淋巴细胞亚群（n = 70名受试者）检测L-选择素和VLA-4整合素。中性粒细胞浓度增加了41.8%（P < 0.001），而表达L-选择素的百分比没有变化（79%）。淋巴细胞浓度增加了61.8%（P < 0.001）。表达L-选择素的T细胞百分比从73.5 +/- 8.9降至68.2 +/- 11.4%（P < 0.001）；表达L-选择素的自然杀伤细胞和B细胞的合并群体从80.4 +/- 22.5降至62.7 +/- 25.8%（P < 0.001）。运动前和运动后几乎所有淋巴细胞均表达VLA-4整合素。L-选择素阳性细胞比例的下降可能是由于1）L-选择素的脱落，2）L-选择素阴性亚群的选择性进入，或3）L-选择素阳性亚群的选择性清除。
