Jajtner Adam R, Hoffman Jay R, Townsend Jeremy R, Beyer Kyle S, Varanoske Alyssa N, Church David D, Oliveira Leonardo P, Herrlinger Kelli A, Radom-Aizik Shlomit, Fukuda David H, Stout Jeffrey R
Institute of Exercise Physiology and Wellness, University of Central Florida, Orlando, Florida.
Department of Exercise Physiology, Kent State University, Kent, Ohio.
Physiol Rep. 2016 Dec;4(24). doi: 10.14814/phy2.13058.
This study examined the effect of resistance exercise on the production, recruitment, percentage, and adhesion characteristics of granulocytes with and without polyphenol (PPB) supplementation. Thirty-eight untrained men were randomized into three groups: PPB (n = 13, 21.8 ± 2.5 years, 171.2 ± 5.5 cm, 71.2 ± 8.2 kg), placebo (PL; n = 15, 21.6 ± 2.5 years, 176.5 ± 4.9 cm, 84.0 ± 15.7 kg), or control (CON; n = 10, 23.3 ± 4.3 years, 173.7 ± 12.6 cm, 77.3 ± 16.3 kg). Blood samples were obtained pre (PRE), immediately (IP), 1 h (1H), 5 h (5H), 24 h (24H), 48 h (48H), and 96 h (96H) postresistance exercise (PPB/PL) or rest (CON). Fine-needle biopsies were obtained from the vastus lateralis at PRE, 1H, 5H, and 48H. Plasma concentrations and intramuscular content of interleukin-8 (IL-8), granulocyte (G-CSF), and granulocyte-macrophage colony stimulating factor (GM-CSF) were analyzed via multiplex assays. Changes in relative number of circulating granulocytes and adhesion receptor (CD11b) were assessed using flow cytometry. Intramuscular IL-8 was significantly elevated at 1H, 5H, and 48H (P < 0.001). Area under the curve analysis indicated a greater intramuscular IL-8 content in PL than PPB (P = 0.011). Across groups, circulating G-CSF was elevated from PRE at IP (P < 0.001), 1H (P = 0.011), and 5H (P = 0.025), while GM-CSF was elevated at IP (P < 0.001) and 1H (P = 0.007). Relative number of granulocytes was elevated at 1H (P < 0.001), 5H (P < 0.001), and 24H (P = 0.005, P = 0.006) in PPB and PL, respectively. Across groups, granulocyte CD11b expression was upregulated from PRE to IP (P < 0.001) and 1H (P = 0.015). Results indicated an increase in circulating CD11b on granulocytes, and IL-8 within the muscle following intense resistance exercise. Polyphenol supplementation may attenuate the IL-8 response, however, did not affect granulocyte percentage and adhesion molecule expression in peripheral blood following resistance exercise.
本研究调查了阻力训练对补充和未补充多酚（PPB）情况下粒细胞的生成、募集、百分比及黏附特性的影响。38名未经训练的男性被随机分为三组：PPB组（n = 13，年龄21.8±2.5岁，身高171.2±5.5厘米，体重71.2±8.2千克）、安慰剂组（PL；n = 15，年龄21.6±2.5岁，身高176.5±4.9厘米，体重84.0±15.7千克）或对照组（CON；n = 10，年龄23.3±4.3岁，身高173.7±12.6厘米，体重77.3±16.3千克）。在进行阻力训练（PPB/PL）或休息（CON）前（PRE）、训练后即刻（IP）、1小时（1H）、5小时（5H）、24小时（24H）、48小时（48H）和96小时（96H）采集血样。在PRE、1H、5H和48H从股外侧肌获取细针活检样本。通过多重检测分析白细胞介素-8（IL-8）、粒细胞集落刺激因子（G-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）的血浆浓度和肌肉内含量。使用流式细胞术评估循环粒细胞和黏附受体（CD11b）相对数量的变化。肌肉内IL-8在1H、5H和48H显著升高（P < 0.001）。曲线下面积分析表明PL组肌肉内IL-8含量高于PPB组（P = 0.011）。在所有组中，循环G-CSF在IP（P < 0.001）、1H（P = 0.011）和5H（P = 0.025）时较PRE升高，而GM-CSF在IP（P < 0.001）和1H（P = 0.007）时升高。PPB组和PL组中粒细胞相对数量分别在1H（P < 0.001）、5H（P < 0.001）和24H（P = 0.005，P = 0.006）时升高。在所有组中，粒细胞CD11b表达从PRE到IP（P < 0.001）和1H（P = 0.015）上调。结果表明，高强度阻力训练后粒细胞表面循环CD11b增加，肌肉内IL-8增加。补充多酚可能会减弱IL-8反应，然而，对阻力训练后外周血中粒细胞百分比和黏附分子表达没有影响。
