通过顺式作用DNA序列控制溶组织内阿米巴中ferredoxin和Gal/GalNAc凝集素基因的表达。
Control of ferredoxin and Gal/GalNAc lectin gene expression in Entamoeba histolytica by a cis-acting DNA sequence.
作者信息
Gilchrist C A, Mann B J, Petri W A
机构信息
Department of Internal Medicine, University of Virginia, Charlottesville 22908, USA.
出版信息
Infect Immun. 1998 May;66(5):2383-6. doi: 10.1128/IAI.66.5.2383-2386.1998.
Abstract
The ferredoxin (fdx) and lectin (hgl5) promoters of Entamoeba histolytica contain the DNA sequence motif TATTCTATT (URE3). Previously we showed that mutation of the URE3 motif in the hgl5 lectin promoter results in an increase in promoter reporter activity. Mutation of this motif in the fdx promoter led to a 40-to-50% decrease in fdx promoter activity as measured by reporter gene activity and abundance of mRNA. E. histolytica nuclear proteins exhibited sequence-specific binding to the URE3 motif in electrophoretic mobility shift assays. These results support the regulation of both ferredoxin and lectin promoters by a nuclear protein(s) which recognizes the URE3 motif.
摘要
溶组织内阿米巴的铁氧化还原蛋白（fdx）启动子和凝集素（hgl5）启动子含有DNA序列基序TATTCTATT（URE3）。先前我们表明，hgl5凝集素启动子中URE3基序的突变导致启动子报告基因活性增加。fdx启动子中该基序的突变导致通过报告基因活性和mRNA丰度测量的fdx启动子活性降低40%至50%。在电泳迁移率变动分析中，溶组织内阿米巴核蛋白表现出与URE3基序的序列特异性结合。这些结果支持铁氧化还原蛋白和凝集素启动子均受识别URE3基序的一种或多种核蛋白的调控。
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