van 't Wout A B, Blaak H, Ran L J, Brouwer M, Kuiken C, Schuitemaker H
Department of Clinical Viro-Immunology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands.
J Virol. 1998 Jun;72(6):5099-107. doi: 10.1128/JVI.72.6.5099-5107.1998.
To investigate the temporal relationship between human immunodeficiency virus type 1 (HIV-1) replicative capacity and syncytium-inducing (SI) phenotype, biological and genetic characteristics of longitudinally obtained virus clones from two HIV-1-infected individuals who developed SI variants were studied. In one individual, the emergence of rapidly replicating SI and non-syncytium-inducing (NSI) variants was accompanied by a loss of the slowly replicating NSI variants. In the other subject, NSI variants were always slowly replicating, while the coexisting SI variants showed an increase in the rate of replication. Irrespective their replicative capacity, the NSI variants remained present throughout the infection in both individuals. Phylogenetic analysis of the V3 region showed early branching of the SI variants from the NSI tree. Successful SI conversion seemed a unique event since no SI variants were found among later-stage NSI variants. This was also confirmed by the increasing evolutionary distance between the two subpopulations. At any time point during the course of the infection, the variation within the coexisting SI and NSI populations did not exceed 2%, indicating continuous competition within each viral subpopulation.
为了研究1型人类免疫缺陷病毒（HIV-1）复制能力与合胞体诱导（SI）表型之间的时间关系，我们对两名出现SI变异体的HIV-1感染者纵向获取的病毒克隆的生物学和遗传学特征进行了研究。在一名个体中，快速复制的SI变异体和非合胞体诱导（NSI）变异体的出现伴随着缓慢复制的NSI变异体的消失。在另一名受试者中，NSI变异体始终复制缓慢，而共存的SI变异体则显示出复制速率增加。无论其复制能力如何，NSI变异体在两名个体的整个感染过程中均持续存在。对V3区域的系统发育分析表明，SI变异体在NSI谱系树中早期分支。成功的SI转换似乎是一个独特事件，因为在后期的NSI变异体中未发现SI变异体。两个亚群之间不断增加的进化距离也证实了这一点。在感染过程中的任何时间点，共存的SI和NSI群体内的变异不超过2%，表明每个病毒亚群内存在持续竞争。
