Rumery R E, Blandau R J
J Morphol. 1976 Jul;149(3):421-36. doi: 10.1002/jmor.1051490309.
Whole ovaries from 16-day fetal mice were cultured for 6 to 20 days and then transplanted to the kidneys of ovariectomized adult mice where they remained for one to four weeks. After three weeks in the host's kidneys, many follicles developed within the transplants and became vesiculated. Many of the oocytes within these follicles had formed the first metaphase spindle of meiosis and several had completed the first polar body. Host mice bearing transplants that contained vesiculated follicles showed uterine stimulation and keratinization of their vaginae. However, ovaries that had been in culture for more than ten days before transplantation showed a limited response to the gonadotropins and never matured sufficiently to stimulate the host's reproductive tract. No ovulations occurred in any of the transplants.
取自16日龄胎鼠的完整卵巢被培养6至20天，然后移植到成年去卵巢小鼠的肾脏中，在那里它们停留1至4周。移植到宿主肾脏三周后，移植组织内形成了许多卵泡并形成囊泡。这些卵泡中的许多卵母细胞已经形成了减数分裂的第一次中期纺锤体，有几个已经完成了第一极体的形成。携带含有囊泡卵泡移植组织的宿主小鼠出现子宫刺激和阴道角质化。然而，移植前培养超过十天的卵巢对促性腺激素的反应有限，从未充分成熟到足以刺激宿主的生殖道。所有移植组织均未发生排卵。
