Arakawa H, Kuma K, Yasuda M, Furusawa S, Ekino S, Yamagishi H
Department of Biophysics, Graduate School of Science, Kyoto University, Japan.
J Immunol. 1998 May 1;160(9):4232-41.
Chicken single germinal centers enable us to analyze the postbursal diversifications of B cells due to their easy isolation. Germinal center formation has peaked by day 7 of primary responses and begins to wane 14 days after immunization. To detail the kinetics of Ig mutation and selection, we analyzed Ig light chain sequences recovered from single germinal centers at 7 and 11 days postimmunization with an artificial Ag. Our observations show that multiple, Ag-activated B cells migrating into single germinal centers are diversified by gene conversion in the very early phase of the germinal center reaction and are subsequently subjected to point mutations and selection for oligoclonality.
鸡的单个生发中心便于分离，这使我们能够分析法氏囊后B细胞的多样化情况。生发中心在初次免疫反应的第7天达到峰值，免疫后14天开始衰退。为详细了解Ig突变和选择的动力学，我们分析了在免疫人工抗原后第7天和第11天从单个生发中心回收的Ig轻链序列。我们的观察结果表明，多个被抗原激活并迁移到单个生发中心的B细胞，在生发中心反应的早期通过基因转换实现多样化，随后经历点突变并进行寡克隆选择。
