West M L, Ramsdale T E
Centre for Drug Design and Development, University of Queensland, St. Lucia, Australia.
Biomed Pept Proteins Nucleic Acids. 1996;2(3):85-8.
Surface plasmon resonance techniques have been used to examine the kinetics of binding for two RNA fragments to an RNA binding domain of HIV-1 REv. RBE3 RNA elicited an apparent dissociation constant (KD) of 121 nM while RREIIB41-79 RNA exhibited an apparent dissociation constant (KD) of 2.5 nM. The dissociation rates for both RNA fragments were comparable. However, the shorter sequence, RBE3, exhibited considerably slower association kinetics. A series of known inhibitors were assayed against these RNA' and the derived K1's were consistent with those reported in the literature, validating the method for routine inhibitor assays.
表面等离子体共振技术已被用于检测两个RNA片段与HIV-1 Rev的RNA结合结构域的结合动力学。RBE3 RNA的表观解离常数（KD）为121 nM，而RREIIB41-79 RNA的表观解离常数（KD）为2.5 nM。两个RNA片段的解离速率相当。然而，较短的序列RBE3表现出明显较慢的缔合动力学。针对这些RNA检测了一系列已知抑制剂，所得的K1值与文献报道一致，验证了该用于常规抑制剂检测的方法。
