Koshikawa N, Imai T, Takahashi I, Yamauchi M, Sawada S, Kansaku A
Department of Pharmacology, Nihon University School of Dentistry, Tokyo, Japan.
Methods Find Exp Clin Pharmacol. 1998 Jan-Feb;20(1):47-51. doi: 10.1358/mf.1998.20.1.485631.
Effects of the Kampo (Chinese herbal) medicines Hochu-ekki-to Yoku-kan-san and Saiko-ka-ryukotsu-borei-to, on behavioral despair and acetic acid-induced writhing were studied in mice. The Kampo medicines were administered for 14 consecutive days in the drinking water. In a behavioral despair study, mice were placed in a water tank containing a water wheel from which there was no escape for 15 min and the number of wheel rotations was counted as escape attempts. In accord with previous studies, imipramine (10 mg/kg i.p.) given daily for 3 days 10 min before testing markedly increased the number of wheel rotations. Hochu-ekki-to (60, 150 and 300 mg/kg/day) similarly increased the number of wheel rotations but the effect was not dose-dependent. Yoku-kan-san markedly increased the number of wheel rotations at lower doses (60 and 150 mg/kg/day), but decreased the number at the highest dose (300 mg/kg/day). Saiko-ka-ryukotsu-borei-to also increased the number of wheel rotations at the lowest does (60 mg/kg/day) but decreased the number at higher doses (150 and 300 mg/kg/day). In an antinociception study, all these Kampo medicines reduced the number of acetic acid-induced writhings, although the effect of Saiko-ka-ryukotsu-borei-to were not dose-dependent. These results suggests that these Kampo medicines may have antidepressive and antinociceptive properties.
研究了汉方（中药）制剂补中益气汤、逍遥散和柴胡加龙骨牡蛎汤对小鼠行为绝望和乙酸诱导扭体反应的影响。将这些汉方制剂连续14天添加到饮用水中给药。在行为绝望实验中，将小鼠置于一个装有水车且无法逃脱的水箱中15分钟，水车转动次数作为逃脱尝试次数进行计数。与先前研究一致，在测试前10分钟每天腹腔注射3天的丙咪嗪（10毫克/千克）显著增加了水车转动次数。补中益气汤（60、150和300毫克/千克/天）同样增加了水车转动次数，但效果不呈剂量依赖性。逍遥散在较低剂量（60和150毫克/千克/天）时显著增加了水车转动次数，但在最高剂量（300毫克/千克/天）时却减少了转动次数。柴胡加龙骨牡蛎汤在最低剂量（60毫克/千克/天）时也增加了水车转动次数，但在较高剂量（150和300毫克/千克/天）时减少了转动次数。在抗伤害感受实验中，所有这些汉方制剂都减少了乙酸诱导的扭体次数，尽管柴胡加龙骨牡蛎汤的效果不呈剂量依赖性。这些结果表明，这些汉方制剂可能具有抗抑郁和抗伤害感受特性。
