Wang Xin Q, Takahashi Takashi, Zhu Shi-Jie, Moriya Junji, Saegusa Seiichiro, Yamakawa Jun'ichi, Kusaka Kazuya, Itoh Tohru, Kanda Tsugiyasu
Department of General Medicine, Kanazawa Medical University, Ishikawa, Japan.
Evid Based Complement Alternat Med. 2004 Sep 1;1(2):203-206. doi: 10.1093/ecam/neh020.
We aimed to evaluate the effect of a Japanese herbal medicine, Hochu-ekki-to (TJ-41), on daily activity in a murine model of chronic fatigue syndrome (CFS). CFS was induced by repeated injection of Brucella abortus (BA) antigen every 2 weeks. TJ-41 was orally administered to mice in a dose of 500 mg/kg/day for 1 week before injecting BA and for 4 weeks thereafter. We evaluated daily running activity in mice receiving TJ-41 as compared with that in untreated mice. Survival of both mouse groups was also monitored during the observation period. Body weight (BW), spleen weight (SW), SW/ BW ratio and expression levels of interleukin-10 (IL-10) mRNA in spleen were determined in both groups at the time of sacrifice. The daily activity was significantly higher in the treated group than in the control. Two mice in the untreated group died 2 days after the second injection of BA, whereas no mice in the group treated with TJ-41 died. The SW and SW/BW ratio were significantly lower in the treated mice than in the control. Suppressed IL-10 mRNA levels were observed in the spleens of the mice treated with TJ-41. Our data suggest that Hochu-ekki-to might possess an inhibitory effect on the marked decrease in running activity following BA injection.
我们旨在评估一种日本草药补中益气汤（TJ - 41）对慢性疲劳综合征（CFS）小鼠模型日常活动的影响。通过每2周重复注射流产布鲁氏菌（BA）抗原诱导CFS。在注射BA前1周及之后4周，以500 mg/kg/天的剂量给小鼠口服TJ - 41。我们评估了接受TJ - 41的小鼠与未治疗小鼠的日常跑步活动情况。在观察期内还监测了两组小鼠的存活情况。在处死时测定了两组小鼠的体重（BW）、脾脏重量（SW）、SW/BW比值以及脾脏中白细胞介素10（IL - 10）mRNA的表达水平。治疗组的日常活动明显高于对照组。未治疗组的两只小鼠在第二次注射BA后2天死亡，而接受TJ - 41治疗的组中没有小鼠死亡。治疗组小鼠的SW和SW/BW比值明显低于对照组。在接受TJ - 41治疗的小鼠脾脏中观察到IL - 10 mRNA水平受到抑制。我们的数据表明，补中益气汤可能对BA注射后跑步活动的显著下降具有抑制作用。
