Hellsten Y, Sjödin B, Richter E A, Bangsbo J
Copenhagen Muscle Research Centre, August Krogh Institute, Denmark.
Am J Physiol. 1998 Apr;274(4):E600-6. doi: 10.1152/ajpendo.1998.274.4.E600.
The exchange of purines in exercised and rested muscle and their relation to muscle ATP levels after intense intermittent exercise were investigated. Seven subjects performed one-legged knee extensor exercise on the following two occasions: without (control; C) and with (high purines; HP) additional arm exercise. There was a greater net release of hypoxanthine by the exercised muscle during the recovery period in HP compared with C [185 +/- 44 vs. 101 +/- 30 (SE) mumol/kg muscle; P < 0.05]. During recovery, the arterial urate concentration was higher in HP compared with C (peak: 585 +/- 48 vs. 355 +/- 20 mumol/l; P < 0.05). The exercised but not the rested muscle extracted a marked amount of urate (330 mumol/kg muscle) from plasma in the HP trial. Muscle ATP levels after 90 min of recovery in HP were lower than at rest (24.3 +/- 0.6 vs. 20.1 +/- 1.1 mmol/kg dry wt). The present data suggest that a single session of long-term high-intensity intermittent exercise causes a significant release of purines from the muscle into blood, which contributes to a sustained lowered level of the muscle ATP concentration. Furthermore, intensely exercised muscle extracts urate when plasma urate is elevated, an event that may be of importance for the replenishment of oxidized muscle urate stores.
研究了高强度间歇运动后，运动肌肉和休息肌肉中嘌呤的交换情况及其与肌肉ATP水平的关系。七名受试者在以下两种情况下进行单腿膝关节伸展运动：无额外手臂运动（对照组；C）和有额外手臂运动（高嘌呤组；HP）。与C组相比，HP组运动肌肉在恢复期间次黄嘌呤的净释放量更大[185±44 vs. 101±30（SE）μmol/kg肌肉；P<0.05]。恢复期间，HP组的动脉尿酸盐浓度高于C组（峰值：585±48 vs. 355±20 μmol/l；P<0.05）。在HP试验中，运动的肌肉而非休息的肌肉从血浆中摄取了大量尿酸盐（330 μmol/kg肌肉）。HP组恢复90分钟后肌肉ATP水平低于休息时（24.3±0.6 vs. 20.1±1.1 mmol/kg干重）。目前的数据表明，单次长期高强度间歇运动导致肌肉中嘌呤大量释放到血液中，这导致肌肉ATP浓度持续降低。此外，当血浆尿酸盐升高时，剧烈运动的肌肉会摄取尿酸盐，这一事件可能对补充氧化的肌肉尿酸盐储备很重要。
