Wesley R D, Mengeling W L, Lager K M, Clouser D F, Landgraf J G, Frey M L
Virology Swine Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
J Vet Diagn Invest. 1998 Apr;10(2):140-4. doi: 10.1177/104063879801000204.
The suitability of restriction fragment length polymorphism (RFLP) analysis for differentiating a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine strain from other North American field strains was investigated. Open reading frame 5 nucleotide sequence data of the vaccine virus, its parent strain VR-2332, and 22 other strains of PRRSV included in this study indicated that 3 restriction enzyme gel patterns characterize the vaccine virus and the parent strain genotype. The combined 3 RFLP patterns differentiate the vaccine and parent virus from other PRRSV strains. This test will be a valuable tool in epidemiologic studies and will be useful in identifying individual strains in cases of multistrain PRRSV infections.
研究了限制性片段长度多态性（RFLP）分析用于区分猪繁殖与呼吸综合征病毒（PRRSV）疫苗株与其他北美野毒株的适用性。本研究中疫苗病毒、其亲本毒株VR-2332以及其他22株PRRSV的开放阅读框5核苷酸序列数据表明，3种限制性内切酶凝胶图谱可表征疫苗病毒和亲本毒株的基因型。这3种RFLP图谱组合可将疫苗病毒和亲本病毒与其他PRRSV毒株区分开来。该检测将成为流行病学研究中的一项有价值工具，并且在多毒株PRRSV感染病例中识别单个毒株时将很有用。
