Xia Da-Song, Chang Tong, Huang Xin-Yi, Tian Xiao-Xiao, Wang Tao, Cui Xing-Yang, Luo Ling-Zhi, Cai Xue-Hui, Yang Yong-Bo, An Tong-Qing
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Heilongjiang Veterinary Biopharmaceutical Engineering Technology Research Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Transbound Emerg Dis. 2023 Sep 12;2023:9929573. doi: 10.1155/2023/9929573. eCollection 2023.
Porcine reproductive and respiratory syndrome (PRRS), which causes reproductive failure in sows and respiratory symptoms in piglets, poses a significant threat to the global pig industry. PRRS virus (PRRSV) variants continue to emerge and spread among pigs. NADC34-like PRRSV has been imported into China in recent years and has shown potential as an endemic strain, which is of great concern. In this study, a NADC34-like PRRSV, named HLJ13 strain, was isolated from a farm where pigs experienced respiratory symptoms and abortions. Genomic analysis revealed that the HLJ13 strain was a potential recombinant of NADC34-like and NADC30-like strains, and the restriction fragment length polymorphism of HLJ13 was a novel pattern that was not yet listed. In the PRRSV HLJ13-inoculated group, the piglets showed mild clinical symptoms, such as persistent fever, and showed histopathological lesions in the lungs, and the virus was detectable at 3 and 7 days postinoculation in anal and nasal swabs, respectively. Recombination analysis revealed that interlineage recombinant events were detected in 8 out of 27 Chinese NADC34-like PRRSVs. Phylogenetic analysis showed that Chinese NADC34-like PRRSVs were distributed in two clades of lineage 1, and Chinese NADC34-like PRRSVs showed different N-glycosylation modifications in glycoproteins, especially in GP3 and GP5. These findings shed light on the genomic characteristics and pathogenicity of the NADC34-like PRRSV in China.
猪繁殖与呼吸综合征（PRRS）可导致母猪繁殖失败和仔猪出现呼吸道症状，对全球养猪业构成重大威胁。PRRS病毒（PRRSV）变种不断出现并在猪群中传播。近年来，类NADC34 PRRSV已传入中国，并显示出成为地方流行毒株的潜力，这引起了极大关注。在本研究中，从一个猪出现呼吸道症状和流产的猪场分离出一株类NADC34 PRRSV，命名为HLJ13株。基因组分析表明，HLJ13株是类NADC34和类NADC30毒株的潜在重组体，且HLJ13的限制性片段长度多态性是一种尚未列出的新模式。在接种PRRSV HLJ13的组中，仔猪表现出轻度临床症状，如持续发热，并在肺部出现组织病理学病变，且分别在接种后3天和7天在肛门和鼻拭子中检测到病毒。重组分析显示，在27株中国类NADC34 PRRSV中有8株检测到株间重组事件。系统发育分析表明，中国类NADC34 PRRSV分布在谱系1的两个分支中，且中国类NADC34 PRRSV在糖蛋白中表现出不同的N-糖基化修饰，尤其是在GP3和GP5中。这些发现揭示了中国类NADC34 PRRSV的基因组特征和致病性。
