mts1（S100A4）基因是否参与了由γ-干扰素调节的转移过程？

Is mts1(S100A4) gene involved in the metastatic process modulated by gamma-interferon?

作者信息

Chiaramonte R, Fasola S, Lollini P L, De Giovanni C, Comi P

机构信息

Dipartimento di Scienze e Tecnologie Biomediche, LITA, Università degli Studi di Milano, Italia.

出版信息

Pathobiology. 1998;66(1):38-40. doi: 10.1159/000027993.

DOI:10.1159/000027993

PMID:9577965

Abstract

This study investigates the involvement of mts1 (S100A4) in the metastatic process. We have evaluated the levels of mts1 gene in murine cancer cells following modulation of the metastatic ability. We report here that modulation of the expression of mts1 gene in cells derived from a breast murine adenocarcinoma (TS/A) did not correlate with metastatic ability either when pretreated in vitro with gamma-interferon (gamma-IFN) or following transfection with gamma-IFN gene. In another series of experiments we treated the cell lines B16-A and B78H1, both derived from B16 melanoma, with gamma-IFN. An increased expression of mts1 was found only in B16-A, but not B78H 1, correlated with a strong increase in metastatic activity of B16-A clone upon gamma-IFN treatment.

摘要

本研究调查了mts1（S100A4）在转移过程中的作用。我们评估了转移性能力受到调节后鼠癌细胞中mts1基因的水平。我们在此报告，来自乳腺鼠腺癌（TS/A）的细胞中，mts1基因表达的调节与转移能力无关，无论是在体外先用γ-干扰素（γ-IFN）预处理，还是用γ-IFN基因转染后。在另一系列实验中，我们用γ-IFN处理了均源自B16黑色素瘤的细胞系B16-A和B78H1。仅在B16-A中发现mts1表达增加，而在B78H1中未发现，这与γ-IFN处理后B16-A克隆转移活性的强烈增加相关。