Lakshmi M S, Parker C, Sherbet G V
Cancer Research Unit, Medical School, University of Newcastle upon Tyne.
Anticancer Res. 1993 Mar-Apr;13(2):299-303.
The nm23 and mts1 genes are associated with the expression of the metastatic phenotype. We have shown previously that modulation of metastatic behaviour produces parallel changes in the expression of these genes and that the expression of the two genes is co-regulated. Here we show that modulation of gene expression affects the process of tubulin polymerisation. B16 melanoma cell lines F1 and ML8 were treated with alpha melanocyte stimulating hormone (MSH) and all-trans retinoic acid (RA) respectively. MSH reduced the proportion of nm23+ and increased mts1+ F1 cells, with a 55% decrease in the ratio nm23:mts1. In parallel, MSH increased the expression of depolymerised form of tubulin in these cells. Treatment of ML8 cells with RA decreased mts1 positivity to a greater extent that nm23 positivity and the nm23:mts1 ratio increased by 70% and, in parallel, reduced the expression of depolymerised form of tubulin. These data suggest that nm23 and mts1 gene expression regulates the biological behaviour of the tumour cell and confer on it invasive and metastasizing properties by affecting the state of tubulin polymerisation.
nm23和mts1基因与转移表型的表达相关。我们之前已经表明，转移行为的调节会使这些基因的表达产生平行变化，并且这两个基因的表达是共同调控的。在此我们表明，基因表达的调节会影响微管蛋白聚合过程。分别用α - 黑素细胞刺激素（MSH）和全反式维甲酸（RA）处理B16黑色素瘤细胞系F1和ML8。MSH降低了nm23阳性F1细胞的比例并增加了mts1阳性F1细胞的比例，nm23:mts1的比值下降了55%。同时，MSH增加了这些细胞中解聚形式微管蛋白的表达。用RA处理ML8细胞，mts1阳性率的降低幅度大于nm23阳性率，nm23:mts1的比值增加了70%，同时，解聚形式微管蛋白的表达降低。这些数据表明，nm23和mts1基因表达调节肿瘤细胞的生物学行为，并通过影响微管蛋白聚合状态赋予其侵袭和转移特性。
