Del Aguila C, Navajas R, Gurbindo D, Ramos J T, Mellado M J, Fenoy S, Muñoz Fernandez M A, Subirats M, Ruiz J, Pieniazek N J
Sec. Parasitology, Fac. CCEE y Técnicas, Univ. San Pablo CEU, Madrid, Spain.
J Eukaryot Microbiol. 1997 Nov-Dec;44(6):84S-85S. doi: 10.1111/j.1550-7408.1997.tb05798.x.
A prospective study was carried out to determine the prevalence rates of microsporidiosis and other enteroparasites in HIV-positive children in the Madrid area. HIV-positive pediatric patients from three hospitals were enrolled in the study. A total of 293 samples (158 stool and 127 urine) were collected from 83 children whose mean age was 6.3 years and had a mean CD4 count of 504.7/mm3 (range 1-2,220/mm3), 48 of whom suffered diarrhea at the time of the study. Microsporidia identification was investigated in stool and urine samples using Weber's chromotrope-based stain, IIF and PCR species-specific tests. Enteric parasites were identified in 32.5% of the children. Cryptosporidium sp. was the most common parasite encountered (14.4%), followed by Blastocytis sp. (9.6%) and Giardia duodenalis (8.4%). Microsporidia was only found in the stools of one child (1.2% of total and 2% of those with diarrhea) and Enterocytozoon bieneusi was demonstrated by PCR. The patient was 10 years old, presented non-chronic diarrhea and his CD4 count was 298/mm3. These data differ from those previously reported by us in HIV-positive adults (13.9%) in the same area, although this group showed more severely depressed CD4 lymphocyte counts than children. New epidemiological studies should be carried out to elucidate whether additional risk factors exist between these groups.
开展了一项前瞻性研究，以确定马德里地区艾滋病毒阳性儿童的微孢子虫病和其他肠道寄生虫的患病率。来自三家医院的艾滋病毒阳性儿科患者被纳入该研究。共从83名儿童中收集了293份样本（158份粪便和127份尿液），这些儿童的平均年龄为6.3岁，平均CD4细胞计数为504.7/mm³（范围为1 - 2220/mm³），其中48名儿童在研究时患有腹泻。使用基于Weber变色染料的染色法、间接免疫荧光法（IIF）和PCR种特异性检测法对粪便和尿液样本中的微孢子虫进行鉴定。32.5%的儿童检测出肠道寄生虫。隐孢子虫属是最常见的寄生虫（14.4%），其次是芽囊原虫（9.6%）和十二指肠贾第虫（8.4%）。仅在一名儿童的粪便中发现了微孢子虫（占总数的1.2%，腹泻儿童中的2%），并通过PCR检测出了微小隐孢子虫。该患者为10岁，患有非慢性腹泻，其CD4细胞计数为298/mm³。这些数据与我们之前在同一地区艾滋病毒阳性成人中报告的数据（13.9%）不同，尽管该组CD4淋巴细胞计数比儿童更低。应开展新的流行病学研究，以阐明这些群体之间是否存在其他风险因素。
