Moseley H L, Whaley K
Department of Pathology, Western Infirmary, Glasgow.
J Clin Lab Immunol. 1979 Apr;2(1):9-13.
Using indirect immunofluorescence, three regulatory proteins of the complement system, C1-inhibitor (C1-INH), C3b inactivator (C3bINA) and beta 1H globulin have been detected in the glomeruli of patients with glomerulonephritis associated with complement activation. C1-INH and beta 1H were found frequently but C3bINA was rarely detected (only 14 biopsies). beta 1H globulin and C3b inactivator which modulate C3b activity were never found in the absence of C3, but C1-INH was sometimes found in the absence of C1s. The patterns for staining for C1s and C1-INH, C3 and beta 1H were almost identical suggesting that the regulatory proteins are binding to the proteins they regulate, as has been demonstrated in vitro. Thus, in tissues undergoing complement-mediated tissue damage, the extent of complement activation is controlled by the normal regulatory mechanisms.
采用间接免疫荧光法，在与补体激活相关的肾小球肾炎患者的肾小球中检测到补体系统的三种调节蛋白，即C1抑制物（C1-INH）、C3b灭活剂（C3bINA）和β1H球蛋白。C1-INH和β1H经常被发现，但C3bINA很少被检测到（仅14例活检）。调节C3b活性的β1H球蛋白和C3b灭活剂在无C3时从未被发现，但C1-INH有时在无C1s时被发现。C1s和C1-INH、C3和β1H的染色模式几乎相同，这表明调节蛋白正在与其所调节的蛋白结合，正如体外实验所证实的那样。因此，在经历补体介导的组织损伤的组织中，补体激活的程度受正常调节机制控制。
