Sasse M, Krüger M, Schade R, Hlinak A
Institut für Virologie, Fachbereich Veterinärmedizin (Mitte), Freie Universität Berlin.
Berl Munch Tierarztl Wochenschr. 1998 Apr;111(4):121-6.
The immunisation of mammals with LPS- and lipid A-antigens leads to the production of specific antibodies. In the present study, we describe the generation and preparation of antibodies from egg yolks of immunized chickens. Egg yolk antibodies were raised by immunizing laying hens with five LPS- and three lipid A-preparations from various gram-negative bacteria species in different immunisation protocols. Antibodies from collected egg yolks were extracted and purified by means of several standard methods. The purity of antibody-preparations was measured and compared by SDS-PAGE. Specific antibodies were assayed by two different EIA procedures. It could be shown that the immunisation of hens with LPS- and lipid A-Antigens resulted in the production of specific egg yolk antibodies, regardless of immunisation-scheme and amount of antigen.
用脂多糖（LPS）和脂多糖A抗原对哺乳动物进行免疫会导致产生特异性抗体。在本研究中，我们描述了从免疫鸡的蛋黄中产生和制备抗体的过程。通过采用不同的免疫方案，用来自多种革兰氏阴性菌的五种脂多糖制剂和三种脂多糖A制剂对产蛋母鸡进行免疫，从而产生蛋黄抗体。通过几种标准方法从收集的蛋黄中提取和纯化抗体。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测量并比较抗体制剂的纯度。通过两种不同的酶免疫分析（EIA）程序检测特异性抗体。结果表明，无论免疫方案和抗原量如何，用脂多糖和脂多糖A抗原免疫母鸡都会产生特异性蛋黄抗体。
