Terzolo H R, Sandoval V E, Caffer M I, Terragno R, Alcain A
INTA EEA Balcarce, Departamento de Producción Animal, Argentina.
Rev Argent Microbiol. 1998 Apr-Jun;30(2):84-92.
Two groups of 6 laying hens were used to produce IgY. In the vaccinated group (V), hens were injected by intramuscular route with two doses of a Salmonella enterica serovar Enteritidis bacterin at 20-day interval. In the control group (T) hens remained unvaccinated. Four IgY extractions were performed on the egg production of both groups. The first two extractions were carried out using the yolks obtained from the eggs produced during the 4th and 5th post-vaccination week (extracts 1V and 1T) and the other two using the ones from the 6th, 7th and 8th week (2V and 2T). Starting from the extracts 1V and 1T other products were obtained by freezing-thawing (1V-A and 1T-A) and simple (1V-B and 1T-B) or double (1V-C and 1T-C) flow capillary dialysis concentration. All these products were compared using an ELISA test specific for the detection of chicken antibodies against flagellar antigens of S. Enteritidis. In this test, V extracts were positive whereas T extracts were negative. The extract 1V was more positive than the extract 2V. The extract 1V-C was the most positive and was therefore selected to be used as an antiserum in the agglutination tests. This extract contained 1.9 g/dl of total proteins, 0.028 g/dl of triglycerides and 0.012 g/dl of cholesterol and showed an electrophoretic pattern characteristic of IgY. The 1T-C extract was used as a negative control in the agglutination tests. Slide somatic and tube flagellar agglutination tests were simultaneously carried out using both IgY extracts and a standard rabbit anti-Salmonella (IgG) sera. Overall 367 strains from the Enterobacteriaceae family were tested together with two other strains belonging to the Vibrionaceae family. The 1V-C extract specifically agglutinated S. Enteritidis strains in the same way as the rabbit sera. This extract also agglutinated other Salmonella strains antigenically related to S. Enteritidis. Salmonella which did not share somatic or flagellar antigens with S. Enteritidis, other different species of the Enterobacteriaceae family and the two strains of the Vibrionaceae family were all negative. None of the strains tested was agglutinated by the 1T-C extract. This paper show that it is possible to use specific IgY to identify S. enterica serovars. The more extended use of IgY for diagnostic purposes may be a convenient way to complement the current use of mammal polyclonal antibodies.
两组各6只产蛋母鸡用于生产卵黄抗体（IgY）。在接种组（V）中，母鸡通过肌肉注射途径，每隔20天接种两剂肠炎沙门氏菌肠炎血清型菌苗。在对照组（T）中，母鸡不进行接种。对两组母鸡所产鸡蛋进行了4次IgY提取。前两次提取使用接种疫苗后第4周和第5周所产鸡蛋的蛋黄（提取物1V和1T），另外两次使用第6、7和8周所产鸡蛋的蛋黄（2V和2T）。从提取物1V和1T开始，通过冻融（1V-A和1T-A）以及简单（1V-B和1T-B）或双重（1V-C和1T-C）流动毛细管透析浓缩获得其他产品。使用针对检测鸡抗肠炎沙门氏菌鞭毛抗原抗体的ELISA试验对所有这些产品进行比较。在该试验中，V组提取物呈阳性，而T组提取物呈阴性。提取物1V比提取物2V更呈阳性。提取物1V-C阳性程度最高，因此被选作凝集试验中的抗血清。该提取物含有1.9 g/dl总蛋白、0.028 g/dl甘油三酯和0.012 g/dl胆固醇，并呈现出IgY特有的电泳图谱。提取物1T-C用作凝集试验的阴性对照。使用IgY提取物和标准兔抗沙门氏菌（IgG）血清同时进行玻片菌体凝集试验和试管鞭毛凝集试验。总共对来自肠杆菌科的367株菌株以及另外两株弧菌科菌株进行了检测。提取物1V-C与兔血清一样能特异性凝集肠炎沙门氏菌菌株。该提取物还能凝集其他与肠炎沙门氏菌抗原相关的沙门氏菌菌株。与肠炎沙门氏菌不具有共同菌体或鞭毛抗原的沙门氏菌、肠杆菌科的其他不同菌种以及两株弧菌科菌株均呈阴性。所检测的菌株均未被提取物1T-C凝集。本文表明，使用特异性IgY来鉴定肠炎沙门氏菌血清型是可行的。将IgY更广泛地用于诊断目的可能是补充当前哺乳动物多克隆抗体使用的一种便捷方法。
