Machemer L, Lorke D
Mutat Res. 1976 Jul;40(3):243-50. doi: 10.1016/0165-1218(76)90050-1.
In a cytogenetic study on the spermatogonia of Chinese hamster, cyclohexylamine (neutral sulphate) was evaluated for mutagenic effects in comparison with an untreated control group and a group treated with the mutagenic compound cyclophosphamide, by assessing spermatogonial metaphases of treated Chinese hamster for chromosomal structural changes. Each test group comprised 8 male hamsters selected at random. Approximately 100 metaphases from each animal were assessed. The doses were 5 X 150 mg cyclohexylamine sulphate (approx. 5 X 102 mg base) per kg body-weight orally, and 5 X 100 mg cyclophosphamide per kg body-weight orally. The individual doses were administered at intervals of 24 h. Preparations were made 24 h after the final treatment, essentially by the method of Hoo and Bowles [10]. Gaps, breaks, fragments, deletions and translocations were assessed as structural changes; frequencies were determined of the metaphases (a) with aberration(s) including gaps, (b)with aberration(s) less gaps and (c)with translocation(s). Aberrations occurred in the untreated negative control group (1.24% incl. gaps, 0.25% without gaps). Translocations were not seen in the untreated group. In the cyclochexylamine group, the frequencies of the aberrant metaphases were sometimes less than in the control group (0.87% including gaps, 0.37% without gaps). Statistically, the results were not significantly different from the control data. No translocations were seen after administration of cyclohexylamine. The positive cyclophosphamide control group clearly differed from the untreated control and from the cyclohexylamine group in the parameters (a) to (c); mainly, the results were highly significantly different from those obtained in the untreated control group. The frequencies of the aberrant metaphases were 3.41% including gaps and 1.99% without gaps. The frequency of the translocations was 0.71% (5 out of 704). Cyclohexylamine sulphate, administered 5 times at 150 mg/kg body-weight orally, had no mutagenic effect, whereas cyclophosphamide, adminstered 5 times at 100 mg/kg body-weight orally, had a chromosome-damaging effect on Chinese hamster spermatogonia.
在一项关于中国仓鼠精原细胞的细胞遗传学研究中，通过评估经处理的中国仓鼠精原细胞中期的染色体结构变化，将环己胺（中性硫酸盐）与未处理的对照组以及用诱变化合物环磷酰胺处理的组进行比较，以评估其诱变作用。每个测试组随机选取8只雄性仓鼠。对每只动物大约100个中期相进行评估。剂量分别为每千克体重口服5×150毫克环己胺硫酸盐（约5×102毫克碱基）和每千克体重口服5×100毫克环磷酰胺。个体剂量每隔24小时给药一次。在最后一次治疗后24小时制备标本，基本上采用胡和鲍尔斯[10]的方法。将间隙、断裂、片段、缺失和易位评估为结构变化；确定出现以下情况的中期相频率：（a）有包括间隙在内的畸变，（b）有不包括间隙的畸变，（c）有易位。在未处理的阴性对照组中出现了畸变（包括间隙的为1.24%，无间隙的为0.25%）。在未处理组中未观察到易位。在环己胺组中，异常中期相的频率有时低于对照组（包括间隙的为0.87%，无间隙的为0.37%）。从统计学上看，结果与对照数据无显著差异。给予环己胺后未观察到易位。阳性环磷酰胺对照组在参数（a）至（c）方面明显不同于未处理的对照组和环己胺组；主要是，结果与未处理对照组获得的结果高度显著不同。异常中期相的频率包括间隙的为3.41%，无间隙的为1.99%。易位频率为0.71%（704个中有5个）。每千克体重口服150毫克的环己胺硫酸盐给药5次没有诱变作用，而每千克体重口服100毫克的环磷酰胺给药5次对中国仓鼠精原细胞有染色体损伤作用。
