大疱性类天疱疮和寻常型天疱疮：血清血管内皮生长因子、可溶性E选择素和肿瘤坏死因子-α水平的相关行为

Bullous pemphigoid and pemphigus vulgaris: correlated behaviour of serum VEGF, sE-selectin and TNF-alpha levels.

作者信息

Ameglio F, D'Auria L, Cordiali-Fei P, Mussi A, Valenzano L, D'Agosto G, Ferraro C, Bonifati C, Giacalone B

机构信息

Laboratory of Clinical Pathology, San Gallicano Institute, Roma, Italy.

出版信息

J Biol Regul Homeost Agents. 1997 Oct-Dec;11(4):148-53.

PMID:9582615

Abstract

BACKGROUND

Recently, we reported that soluble E-selectin (sE-selectin), an isoform of the cell membrane E-selectin, an adhesion molecule synthesized only by endothelial cells, is significantly increased in sera of the patients with bullous pemphigoid (PB) or pemphigus vulgaris. A significant correlation was also found between the serum sE-selectin levels and the number of skin lesions, suggesting the possible use of this molecule to gauge disease intensity before therapy. One of the sE-selectin inducers is tumor nerosis factor-alpha (TNF-alpha), that is also able to enhance vascular endothelial growth factor (VEGF), a strong endothelium activator.

OBJECTIVE

On the basis of these observations, the present study was conducted to analyze the serum levels of VEGF, sE-selectin, and TNF-alpha in 8 patients with BP (age: 82, range 54-87, 7 males, 1 female) and in 6 patients affected affected with PV (age: 55, range 44-65; 5 males, 1 female) and to verify possible correlations between these variables and the disease activity, In addition, serum sE-selectin levels were measured over time and compared with the serum anti-epithelium antibodies titers.

METHODS

The sE-selectin, VEGF and TNF-alpha levels were measured in the samples by means of commercially available ELISA kit. The same samples were also employed to measure the anti-epithelium antibody titers.

RESULTS

Serum VEGF, sE-selectin and TNF-alpha levels were significantly correlated each other (p at least < 0.01). All three variables were also significantly correlated with the number of lesions (p at least < 0.01). Serum VEGF levels were found increased (median = 178 pg/ml, range 37-595) as compared to 28 healthy controls (median = 135 pg/ml, range 18/269, p < 0.05). Also serum TNF-alpha levels were found increased (median = 5.5 pg/ml, range < 0.1-41.0) as compared to 28 healthy controls (median < 0.1 pg/ml, range < 0.1-5.3), p < 0.01). When the patients were observed over time, serum sE-selectin levels highly correlated with the disease intensity in both dermatoses, although with different regression curves.

CONCLUSIONS

These data further underline the endothelium involvement in these bullous dermatoses and stress the possibility of employing sE-selectin as a non-specific follow-up marker of both BP and PV.

摘要

背景

最近，我们报道了可溶性E-选择素（sE-选择素），一种仅由内皮细胞合成的粘附分子——细胞膜E-选择素的同种型，在大疱性类天疱疮（PB）或寻常型天疱疮患者的血清中显著升高。血清sE-选择素水平与皮肤病变数量之间也存在显著相关性，这表明该分子可能可用于在治疗前评估疾病严重程度。sE-选择素诱导剂之一是肿瘤坏死因子-α（TNF-α），它也能够增强血管内皮生长因子（VEGF），一种强大的内皮激活剂。

目的

基于这些观察结果，本研究旨在分析8例BP患者（年龄：82岁，范围54 - 87岁，7例男性，1例女性）和6例PV患者（年龄：55岁，范围44 - 65岁；5例男性，1例女性）的血清VEGF、sE-选择素和TNF-α水平，并验证这些变量与疾病活动之间的可能相关性。此外，还对血清sE-选择素水平进行了长期测量，并与血清抗上皮细胞抗体滴度进行了比较。

方法

通过市售ELISA试剂盒测量样本中的sE-选择素、VEGF和TNF-α水平。同样的样本也用于测量抗上皮细胞抗体滴度。

结果

血清VEGF、sE-选择素和TNF-α水平彼此显著相关（p至少<0.01）。所有这三个变量也与病变数量显著相关（p至少<0.01）。与28名健康对照者相比，血清VEGF水平升高（中位数 = 每毫升178皮克，范围37 - 595）（中位数 = 每毫升135皮克，范围18 / 269，p < 0.05）。与28名健康对照者相比，血清TNF-α水平也升高（中位数 = 每毫升5.5皮克，范围<0.1 - 41.0）（中位数<0.1皮克/毫升，范围<0.1 - 5.3），p < 0.01）。随着时间的推移观察患者时，血清sE-选择素水平在两种皮肤病中均与疾病严重程度高度相关，尽管回归曲线不同。