Roth G N, Chandra A, Nair M G
Department of Horticulture, Michigan State University, East Lansing 48823, USA.
J Nat Prod. 1998 Apr;61(4):542-5. doi: 10.1021/np970459f.
Bioassay-directed fractionation of ethyl acetate extract from Curcuma longa Linn. rhizomes yielded three curcuminoids, which displayed topoisomerase I and II enzyme inhibition activity. Curcumin III (3) was the most active curcuminoid, inhibiting topoisomerase at 25 micrograms mL-1. Curcumin I (1) and curcumin II (2) inhibited the topoisomerases at 50 micrograms mL-1. Fractionation of the volatile oil from the rhizomes afforded ar-turmerone (4), which displayed mosquitocidal activity with an LD100 of 50 micrograms mL-1 on Aedes aegyptii larvae. Bioassay-directed fractionation of hexane extract from the turmeric leaves yielded labda-8(17),12-diene-15,16 dial (5) with antifungal activity against Candida albicans at 1 micrograms mL-1 and inhibited the growth of Candida kruseii and Candida parapsilosis at 25 micrograms mL-1. In addition, 5 displayed 100% mosquitocidal activity on A. aegyptii larvae at 10 micrograms mL-1.
对姜黄根茎的乙酸乙酯提取物进行生物测定导向的分级分离，得到了三种姜黄素类化合物，它们表现出拓扑异构酶I和II的酶抑制活性。姜黄素III（3）是活性最强的姜黄素类化合物，在25微克/毫升时抑制拓扑异构酶。姜黄素I（1）和姜黄素II（2）在50微克/毫升时抑制拓扑异构酶。对根茎挥发油进行分级分离得到了芳姜黄酮（4），它对埃及伊蚊幼虫表现出杀蚊活性，LD100为50微克/毫升。对姜黄叶片的己烷提取物进行生物测定导向的分级分离，得到了labda-8(17),12-二烯-15,16-二醇（5），它在1微克/毫升时对白色念珠菌具有抗真菌活性，在25微克/毫升时抑制克鲁斯念珠菌和近平滑念珠菌的生长。此外，5在10微克/毫升时对埃及伊蚊幼虫表现出100%的杀蚊活性。
