Lim Huimei, Lee Sook Yee, Ho Lai Yee, Sit Nam Weng
Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar 31900, Malaysia.
Insects. 2023 Jun 1;14(6):512. doi: 10.3390/insects14060512.
Despite ongoing control efforts, the mosquito population and diseases vectored by them continue to thrive worldwide, causing major health concerns. There has been growing interest in the use of botanicals as alternatives to insecticides due to their widespread insecticidal properties, biodegradability, and adaptability to ecological conditions. In this study, we investigated the larvicidal activity and cytotoxicity effects of solvent extracts from three aromatic plants- (turmeric), (hoary basil), and (parsley)-against . Subsequently, we examined the phytochemical composition of the extracts through gas chromatography-mass spectrometry (GC-MS) analysis. Results revealed that the hexane extracts of and exhibited the greatest larvicidal activity with the lowest median lethal concentration (LC) values (<30 µg/mL) at 24 h post-treatment, with the former found to be significantly less toxic towards African monkey kidney (Vero) cells. The GC-MS analysis of the said extract indicated the presence of different classes of metabolites, including phenylpropanoids, very long-chain alkanes, fatty acids and their derivatives, and terpenes, with the most abundant component being methyl eugenol (55.28%), most of which, have been documented for their larvicidal activities. These findings provide valuable insights into the potential use and development of bioinsecticides, particularly from .
尽管一直在进行防治工作，但蚊子种群及其传播的疾病在全球范围内仍持续蔓延，引发了重大的健康问题。由于植物提取物具有广泛的杀虫特性、生物降解性以及对生态条件的适应性，人们对其作为杀虫剂替代品的兴趣与日俱增。在本研究中，我们调查了三种芳香植物（姜黄、灰白罗勒和欧芹）的溶剂提取物对[某种蚊子]的杀幼虫活性和细胞毒性作用。随后，我们通过气相色谱 - 质谱联用（GC - MS）分析检测了提取物的植物化学成分。结果显示，姜黄和灰白罗勒的己烷提取物在处理后24小时表现出最大的杀幼虫活性，最低半数致死浓度（LC）值（<30 µg/mL），且发现前者对非洲绿猴肾（Vero）细胞的毒性显著较低。对上述提取物的GC - MS分析表明存在不同种类的代谢产物，包括苯丙烷类、极长链烷烃、脂肪酸及其衍生物以及萜类，其中最丰富的成分是甲基丁香酚（55.28%），这些成分大多已被证明具有杀幼虫活性。这些发现为生物杀虫剂的潜在用途和开发提供了有价值的见解，特别是来自[这三种植物]的生物杀虫剂。
