脂肪酸介导的马铃薯块茎线粒体解偶联
Fatty acid-mediated uncoupling of potato tuber mitochondria.
作者信息
Saviani E E, Martins I S
机构信息
Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, SP, CEP, Brazil.
出版信息
Biochem Mol Biol Int. 1998 Apr;44(4):833-9. doi: 10.1080/15216549800201882.
The present work examined whether the ATP/ADP carrier, other than the plant uncoupling mitochondrial protein, participates in free fatty acid-mediated uncoupling of potato tuber mitochondria. The basal respiration rate of succinate-energized mitochondria was stimulated by a low concentration of palmitate (20 microM). This uncoupling was reversed by 10 microM carboxyatractyloside and by the subsequent addition of 0.1% bovine serum albumin. The decrease in membrane potential caused by palmitate was suppressed by carboxyatractyloside (1 microM) and, to a lesser degree, by bongkrekate (20 microM). GTP could also reversed this decrease via a carboxyatractyloside-independent mechanism. These results indicate that the ATP/ADP carrier, along with the plant uncoupling mitochondrial protein, participates in the protonophoric action of palmitate in potato tuber mitochondria.
本研究探讨了除植物解偶联线粒体蛋白外，ATP/ADP载体是否参与游离脂肪酸介导的马铃薯块茎线粒体解偶联过程。低浓度棕榈酸（20微摩尔）刺激了琥珀酸供能的线粒体的基础呼吸速率。10微摩尔羧基苍术苷以及随后添加0.1%牛血清白蛋白可逆转这种解偶联。棕榈酸引起的膜电位降低被1微摩尔羧基苍术苷抑制，20微摩尔膨润酸在较小程度上也有抑制作用。GTP也可通过一种不依赖羧基苍术苷的机制逆转这种降低。这些结果表明，ATP/ADP载体与植物解偶联线粒体蛋白一起，参与了棕榈酸在马铃薯块茎线粒体中的质子载体作用。
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