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Novel base-labile protecting groups for 5'-hydroxyl protection in the synthesis of oligonucleotides.

作者信息

Kamaike Kazuo, Namiki Takashi, Kayama Yoshihiro, Kawashima Etsuko

机构信息

School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):151-2. doi: 10.1093/nass/2.1.151.

DOI:10.1093/nass/2.1.151
PMID:12903150
Abstract

The 6-(levulinyloxymethyl)-3-methoxy-2-nitrobenzoyl (LMM0NBz) and 2-(levulinyloxy-methyl)-5-methoxy-4-nitrobenzoyl (LMMpNBz) groups were developed as novel base-labile protection for the 5'-hydroxyl group in solid-phase oligonucleotide synthesis. A comparative study on the utility of LMMoNBz, LMMpNBz, and 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) groups for the synthesis of oligodeoxyribonucleotides, and the synthesis of oligoribonucleotides using the LMMoNBz group in combination with the acid-labile 1-ethoxyethylk (EE) group to protect the 2'-hydroxyl group, are described.

摘要

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