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用于固相寡核苷酸合成中5'-羟基官能团的新型碱不稳定保护基团。

Novel base-labile protecting groups for 5'-hydroxy function in solid-phase oligonucleotide synthesis.

作者信息

Kamaike Kazuo, Namiki Takashi, Kawashima Etsuko

机构信息

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1011-5. doi: 10.1081/NCN-120022725.

DOI:10.1081/NCN-120022725
PMID:14565333
Abstract

The 6-(levulinyloxymethyl)-3-methoxy-2-nitrobenzoyl (LMMoNBz) and 2-(levulinyloxymethyl)-5-methoxy-4-nitrobenzoyl (LMMpNBz) groups were developed as novel base-labile protection for the 5'-hydroxy function in solid-phase oligonucleotide synthesis. A comparative study of the LMMoNBz, LMMpNBz and 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) protecting groups for oligonucleotide synthesis proved strong feasibility for the LMMoNBz group.

摘要

6-(乙酰氧基甲基)-3-甲氧基-2-硝基苯甲酰基（LMMoNBz）和2-(乙酰氧基甲基)-5-甲氧基-4-硝基苯甲酰基（LMMpNBz）基团被开发用作固相寡核苷酸合成中5'-羟基官能团的新型碱不稳定保护基。对用于寡核苷酸合成的LMMoNBz、LMMpNBz和2-(乙酰氧基甲基)-5-硝基苯甲酰基（LMNBz）保护基的比较研究证明了LMMoNBz基团具有很强的可行性。

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Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1011-5. doi: 10.1081/NCN-120022725.
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