通过准分子形成双探针核酸杂交法诱导的芘准分子的最佳构型。

Optimal configuration of pyrene excimer induced by the excimer-forming two-probe nucleic acid hybridization method.

作者信息

Masuko M, Toyoda S, Suwa M, Mitaku S, Shimazu A, Ohtani H

机构信息

Tsukuba Research Laboratory, Hamamatsu Photonics K. K., Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):83-4.

PMID:9586010

Abstract

Excimer-forming two-probe nucleic acid hybridization (ETPH) method with pyrene as a fluorophore enables homogeneous hybridization assays. We examined the effect of linker length between a pyrene residue and a terminal sugar moiety on Tm of hybrids in the presence of 20% dimethylformamide (DMF). The results including those of CD measurements indicated no interaction of pyrene residues with the duplex formed between a target 32-mer and a pyrenemethyliodoacetamide-introduced 16-mer probe (PMIA-P5)/a pyrenebutanoic acid-introduced 16-mer probe (PBuA-P3), which is the best pair of probes for intense excimer emission. This was also supported by a computer-assisted molecular modeling using Insight II and Discover software.

摘要

以芘作为荧光团的准分子形成双探针核酸杂交（ETPH）方法可实现均相杂交分析。我们研究了芘残基与末端糖部分之间的连接子长度对在20%二甲基甲酰胺（DMF）存在下杂交体熔解温度（Tm）的影响。包括圆二色性（CD）测量结果在内的结果表明，芘残基与在靶标32聚体与引入了碘代乙酰氨基芘的16聚体探针（PMIA-P5）/引入了丁酸芘的16聚体探针（PBuA-P3）之间形成的双链体没有相互作用，这是用于强烈准分子发射的最佳探针组合。这也得到了使用Insight II和Discover软件进行的计算机辅助分子建模的支持。