Satoh A, Takai K, Yokoyama S, Takaku H
Department of Industrial Chemistry, Faculty of Technology, Chiba Institute of Technology, Japan.
Nucleic Acids Symp Ser. 1997(37):117-8.
Proteins with unnatural amino acids at specific positions can be produced through cell-free protein synthesis. The synthesis of such molecules can, in principle, be facilitated by improving the codon reading efficiency of the tRNA that inserts the unnatural amino acid. In the present study, we prepared tRNA molecules with 2'-O-methyl nucleosides at the second and third positions of the anticodon and measured their codon-reading efficiencies. The results indicated, contrary to our expectation, that the modification damaged the decoding function completely.
在特定位置含有非天然氨基酸的蛋白质可通过无细胞蛋白质合成来产生。原则上，插入非天然氨基酸的tRNA的密码子阅读效率的提高有助于此类分子的合成。在本研究中，我们制备了在反密码子的第二位和第三位带有2'-O-甲基核苷的tRNA分子，并测量了它们的密码子阅读效率。结果表明，与我们的预期相反，这种修饰完全破坏了解码功能。
