含有G簇的寡核苷酸的四重结构形成及其核酸酶抗性。

Quadruplex formation of oligonucleotides containing G clusters and their nuclease resistance.

作者信息

Shida T, Suda M, Sekiguchi J

机构信息

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.

出版信息

Nucleic Acids Symp Ser. 1997(37):129-30.

PMID:9586033

Abstract

Oligoribonucleotides containing four G clusters, (UGnU)4 (n = 3-5), were synthesized and characterized by electrophoretic analysis and resistance to nucleases. Electrophoretic analyses indicate that the oligomers except for (UG3U)4 can form antiparallel-stranded monomeric quadruplexes in the presence of potassium cation. The oligomers used in this study are protected from endonuclease P1 and venom phosphodiesterase degradation. In addition, the oligomers indicate resistance to nucleases in human serum and E. coli S-100 fraction.

摘要

合成了含有四个G簇的寡核糖核苷酸(UGnU)4(n = 3 - 5)，并通过电泳分析和核酸酶抗性进行了表征。电泳分析表明，除(UG3U)4外，这些寡聚物在钾离子存在下可形成反平行链单体四链体。本研究中使用的寡聚物对内切核酸酶P1和蛇毒磷酸二酯酶的降解具有抗性。此外，这些寡聚物对人血清和大肠杆菌S - 100组分中的核酸酶也具有抗性。