Fukuda K, Vishinuvardhan D, Sekiya S, Kakiuchi N, Shimotohno K, Kumar P K, Nishikawa S
Natioal Institute of Bioscience and Human Technology, Tsukuba Science City, Japan.
Nucleic Acids Symp Ser. 1997(37):237-8.
In order to isolate RNA aptamers that bind specifically to NS3 protease domain (delta NS3) of hepatitis C virus, we carried out in vitro selection procedure using RNA pool that had 30 N random core region. After repeating nine cycles of selections and amplifications, a pool of RNAs that bind specifically to the delta NS3 were selected. A comparative analysis of 45 clones that were isolated from 9th cycle revealed three main classes that contain the conserved loop sequences GANUGGGAC. Moreover, the predominant class of aptamer (class I and III) appear to inhibit the protease activity efficiently.
为了分离出能特异性结合丙型肝炎病毒NS3蛋白酶结构域（δNS3）的RNA适配体，我们使用了具有30个随机N核心区域的RNA文库进行体外筛选。经过九轮筛选和扩增后，筛选出了一组能特异性结合δNS3的RNA。对从第九轮分离出的45个克隆进行的比较分析显示，有三个主要类别包含保守的环序列GANUGGGAC。此外，主要类别的适配体（I类和III类）似乎能有效抑制蛋白酶活性。
