Negishi K, Hata Y, Sanda K, Hayatsu H
Gene Research Center, Okayama University, Japan.
Nucleic Acids Symp Ser. 1997(37):289-90.
We have studied mutagenic specificity of an abasic site by the yeast-transformation procedure using an oligonucleotide containing a single furan-type abasic site. The recipient yeast used was deficient in the major AP endonuclease (apn1). Sequence analysis of the transformants suggested that dATP was incorporated most frequently opposite the abasic site, while dGTP seemed to be incorporated opposite the abasic site in the recipient proficient in apn1. To explore the mechanism of this oligonucleotide transformation, we have also analyzed the transformation with phosphorothioate oligonucleotides with mismatched 3'-end. The results are discussed.
我们通过酵母转化程序，使用含有单个呋喃型无碱基位点的寡核苷酸，研究了无碱基位点的诱变特异性。所使用的受体酵母缺乏主要的AP内切核酸酶（apn1）。对转化体的序列分析表明，dATP最常掺入到与无碱基位点相对的位置，而在apn1功能正常的受体中，dGTP似乎掺入到与无碱基位点相对的位置。为了探究这种寡核苷酸转化的机制，我们还分析了3'-末端错配的硫代磷酸寡核苷酸的转化情况。对结果进行了讨论。
