McCullough N V, Brosseau L M, Vesley D, Vincent J H
University of Minnesota, School of Public Health, Division of Environmental and Occupational Health, Minneapolis 55455, USA.
Am Ind Hyg Assoc J. 1998 Apr;59(4):234-41. doi: 10.1080/15428119891010497.
In preparation for filter efficiency tests and sampler comparison studies, methods of biological aerosol generation, sampling, and filter recovery were modified from previous studies. Methods described include (1) techniques for generating aerosols that reduced nuisance particles to negligible levels and increased the cell culturability of Mycobacterium abscessus by 30%, (2) sampling techniques that lowered the detectable range of biological particle size from 0.65 to 0.45 micron and reduced the sampling flow from the chamber from 28.3 to 1.5 L/min, and (3) development of methods to remove culturable organisms from respirator filter media. These methods were developed for filter challenge tests with M. abscessus and were applied to two other bacteria. They may also have application to a wider variety of organisms and bioaerosol assessments.
在准备过滤器效率测试和采样器比较研究时，生物气溶胶生成、采样和过滤器回收方法较之前的研究有所改进。所描述的方法包括：（1）生成气溶胶的技术，该技术将有害颗粒减少到可忽略不计的水平，并使脓肿分枝杆菌的细胞可培养性提高了30%；（2）采样技术，该技术将生物颗粒大小的可检测范围从0.65微米降低到0.45微米，并将腔室的采样流量从28.3升/分钟降低到1.5升/分钟；（3）开发从呼吸器过滤介质中去除可培养生物的方法。这些方法是为用脓肿分枝杆菌进行过滤器挑战测试而开发的，并应用于另外两种细菌。它们也可能适用于更广泛的生物体和生物气溶胶评估。
