Stahnke N, Blunck W, Schröter W
Pediatr Res. 1976 Sep;10(9):802-6. doi: 10.1203/00006450-197609000-00008.
Fifteen red cell enzyme activities of growth-retarded patients with and without growth hormone (GH) deficiency were investigated before and after GH administration. The 15 enzymes were Hexokinase, phosphoglucomutase, glucose phosphate, isomerase, phosphofructokinase, fructose diphosphate aldolase, glyceraldehyde-3-phosphae dehydrogenase, triosephosphate isomerase, 2,3-diphosphoglycerate mutase, 3-phosphoglycerate kinase, 3-phosphoglycerate mutase, enolase, pyruvate kinase, glycose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, glutathione reducase. Sixty-six subjects were studied: 30 normal control subjects (group N) and 36 patients (aged 5-23 years) with short stature. Complete endocrine evaluation showed 21 (group I) to have GH deficiency (10 patients with isolated GH deficiency) and 15 (group II) to have normal hypothalamic and pituitary function except for two patients with a moderate hypothyroidism. Both had been receiving thyroid hormone treatment for a long time before our studies. All 36 patients were treated with 2 mg human growth hormone intramuscularly for 7 days. Before GH treatment no significant difference was observed between hematologic data in group I (GH deficiency) and group II (no GH deficiency). After GH therapy there was a significant increase in reticulocyte count in both groups of patients with short stature. The mean pretreatment value in group I was 1.294% +/- 0.084 (SEM); the mean post-treatment value was 2.081% +/- 0.287 (SEM)< P less than 0.005. The mean pretreatment value in group II was 1.0% 0.184 (SEM); the mean post-treatment value was 1.407% +/- 0.193 (SEM), P less than 0.01. In group II (no GH deficiency) mean pretreatment erythrocyte enzyme activities were not significantly different from those activities observed in normal control subjects (group N). However, in patients who lacked GH, the pretreatment activities of five red cell enzymes (glucose phosphate isomerase, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, 2,3-diphosphoglycerate mutase, 3-phosphoglycerate kinase) were significantly decreased before GH administration compared with the values in normal control subjects...
对15名生长激素（GH）缺乏和不缺乏的生长发育迟缓患者在给予GH前后进行了15种红细胞酶活性的研究。这15种酶分别是己糖激酶、磷酸葡萄糖变位酶、磷酸葡萄糖异构酶、磷酸果糖激酶、果糖二磷酸醛缩酶、甘油醛-3-磷酸脱氢酶、磷酸丙糖异构酶、2,3-二磷酸甘油酸变位酶、3-磷酸甘油酸激酶、3-磷酸甘油酸变位酶、烯醇化酶、丙酮酸激酶、葡萄糖-6-磷酸脱氢酶、6-磷酸葡糖酸脱氢酶、谷胱甘肽还原酶。共研究了66名受试者：30名正常对照者（N组）和36名身材矮小的患者（年龄5 - 23岁）。完整的内分泌评估显示，21名（I组）存在GH缺乏（10名单纯GH缺乏患者），15名（II组）除2名中度甲状腺功能减退患者外，下丘脑和垂体功能正常。在我们研究之前，这两名患者均已接受长期甲状腺激素治疗。所有36名患者均接受2mg人生长激素肌肉注射，为期7天。在GH治疗前，I组（GH缺乏）和II组（无GH缺乏）的血液学数据未观察到显著差异。GH治疗后，两组身材矮小患者的网织红细胞计数均显著增加。I组治疗前的平均值为1.294%±0.084（标准误）；治疗后的平均值为2.081%±0.287（标准误），P<0.005。II组治疗前的平均值为1.0%±0.184（标准误）；治疗后的平均值为1.407%±0.193（标准误），P<0.01。在II组（无GH缺乏）中，治疗前红细胞酶活性与正常对照者（N组）观察到的活性无显著差异。然而，在缺乏GH的患者中，与正常对照者的值相比，GH给药前五种红细胞酶（磷酸葡萄糖异构酶、磷酸丙糖异构酶、甘油醛-3-磷酸脱氢酶、2,3-二磷酸甘油酸变位酶、3-磷酸甘油酸激酶）的活性显著降低……
