Nemceková E, Nosálóvá G, Franová S
Ustav farmakológic Jeseniovej lekárskej fakulty Univerzity Komenského v Martine, Slovakia.
Bratisl Lek Listy. 1998 Feb;99(2):111-5.
Ambroxol, trans-4-/(2-amino-3,5-dibromobenzyl) amino/cyclohexanol hydrochloride, a drug used to increase surfactant secretion in the lungs, has been reported to be effective in reducing exacerbation of chronic bronchitis and in the protection from inflammatory reactions (Bianchi et al., 1990). The effect of this drug on the defence reflexes of airways remains unknown.
The aim of this study was to follow the changes in the cough reflex after administration of ambroxol in the dose of 10 mg/kg body weight. Ambroxol was administered i.p. and perorally. Further, we investigated the bronchodilatory activity of ambroxol.
In the present study, the effects of ambroxol on the cough reflex in the cats were investigated. The drug was administered intraperitoneally and perorally in the dose of 10 mg/kg body weight. Cough was induced by mechanical stimulation of airways. The effect of ambroxol on the smooth muscles of guinea pig (weight 300-400 g) airways was investigated in vitro. The contractions were induced by carbachol in the concentration of 10(-5) mol/l and by histamine in the same concentration.
The results indicate that ambroxol, when administered intraperitoneally attained a total fall in cough parameters by about 51.6%. Ambroxol, when administered perorally suppressed all cough parameters by about 37.04%. Further, ambroxol has no effect on carbachol-induced contraction of smooth muscles of airways. The histamine-induced contraction of tracheal smooth muscles was significantly reduced after administration of ambroxol in the concentration of 10(-4) mol/l. The lung smooth muscles of guinea pig became relaxed after administration of the drug in the concentrations of 10(-5)-10(-4) mol/l.
The study suggests, that ambroxol has a cough-suppressive effect. More expressive effect of the drug on smooth muscles of airways was found out in case of histamine-induced contraction. (Fig. 6, Ref. 13.)
氨溴索，即反式-4-[(2-氨基-3,5-二溴苄基)氨基]环己醇盐酸盐，是一种用于增加肺表面活性物质分泌的药物，据报道其在减少慢性支气管炎急性发作及预防炎症反应方面有效（比安奇等人，1990年）。该药物对气道防御反射的作用尚不清楚。
本研究的目的是观察给予10mg/kg体重剂量的氨溴索后咳嗽反射的变化。氨溴索通过腹腔注射和口服给药。此外，我们还研究了氨溴索的支气管扩张活性。
在本研究中，研究了氨溴索对猫咳嗽反射的影响。该药物以10mg/kg体重的剂量通过腹腔注射和口服给药。通过气道的机械刺激诱发咳嗽。在体外研究了氨溴索对豚鼠（体重300 - 400g）气道平滑肌的影响。收缩由浓度为10⁻⁵mol/L的卡巴胆碱和相同浓度的组胺诱发。
结果表明，腹腔注射氨溴索后咳嗽参数总体下降约51.6%。口服氨溴索可使所有咳嗽参数降低约37.04%。此外，氨溴索对卡巴胆碱诱导的气道平滑肌收缩没有影响。在给予浓度为10⁻⁴mol/L的氨溴索后，组胺诱导的气管平滑肌收缩明显减弱。在给予浓度为10⁻⁵ - 10⁻⁴mol/L的药物后，豚鼠肺平滑肌松弛。
该研究表明，氨溴索具有镇咳作用。在组胺诱导的收缩情况下，发现该药物对气道平滑肌有更明显的作用。（图6，参考文献13）
