Sutovska M, Franova S, Sutovsky J
Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
Bratisl Lek Listy. 2009;110(5):269-75.
The ATP-sensitive potassium ion channels (K+ATP) are widely expressed on airway sensory nerves that mediate cough and other protective reflexes; dependent on airways smooth muscles (ASM) reactivity.
The study was conducted to determine the involvement of K+ATP in airway sensory nerves activity of different animal species.
In this study we have evaluated the cough reflex inhibiting potential and modulation of ASM reactivity in vitro, by K+ATP opener-pinacidil in guinea pig and cats. The cough in guinea pig was induced by administration, in cats by mechanical stimulation of the airways. ASM reactivity was tested by organ tissue both method of 0.3 M citric acid aerosol at 3 min interval.
K+ATP may be involved in mechanisms of cough reflex, likewise in antitussive activity of several more agents and also on defence reflexes dependent on ASM reactivity in different animal species such as guinea pigs and cats (Fig. 8, Ref. 20). Full Text (Free, PDF) www.bmj.sk.
ATP敏感性钾离子通道（K+ATP）广泛表达于介导咳嗽及其他保护性反射的气道感觉神经上；取决于气道平滑肌（ASM）的反应性。
本研究旨在确定K+ATP在不同动物物种气道感觉神经活动中的作用。
在本研究中，我们通过K+ATP开放剂吡那地尔，评估了豚鼠和猫体外的咳嗽反射抑制潜能以及对ASM反应性的调节。豚鼠的咳嗽通过给药诱导，猫的咳嗽通过气道机械刺激诱导。ASM反应性通过器官组织在间隔3分钟给予0.3M柠檬酸气雾剂的方法进行测试。
1）吡那地尔抑制豚鼠的咳嗽反射，在用K+ATP阻滞剂格列本脲预处理后该作用被拮抗。2）吡那地尔在猫中表现出与可待因相当的镇咳活性，且明显高于二氧丙嗪的效果。3）吡那地尔在豚鼠中显著消除了ASM反应性，且几乎完全被格列本脲拮抗。4）吡那地尔仅在最高浓度的收缩介质中显著减弱了猫的ASM收缩。
K+ATP可能参与咳嗽反射机制，同样也参与多种其他药物的镇咳活性，以及在豚鼠和猫等不同动物物种中依赖于ASM反应性的防御反射（图8，参考文献20）。全文（免费，PDF）www.bmj.sk 。
