Tamura S, Shimozawa N, Suzuki Y, Tsukamoto T, Osumi T, Fujiki Y
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Biochem Biophys Res Commun. 1998 Apr 28;245(3):883-6. doi: 10.1006/bbrc.1998.8522.
Human PEX1 (HsPEX1) is the causative gene for peroxisome-deficiency disorders such as Zellweger syndrome of complementation group I, encoding the peroxin, Pex1p, a member of AAA family. Pex1p tagged with an epitope flag was expressed in wild-type Chinese hamster ovary (CHO) cell, CHO-K1. Pex1p was localized in the cytoplasm, as assessed by immunofluorescent microscopy. Cell-lysate of HsPEX1-transfected CHO-K1 was incubated with in vitro synthesized 35S-labelled Pex6p, an AAA family peroxin. Immunoprecipitation of Pex1p using anti-Pex1p antibody resulted in concomitant recovery of 35S-Pex6p. Conversely, 35S-Pex1p was obtained in immunoprecipitate from CHO-K1 expressing human Pex6p, using anti-Pex6p antibody. These results strongly suggest that Pex1p and Pex6p interact with each other.
人类PEX1（HsPEX1）是过氧化物酶体缺陷疾病的致病基因，如I型互补组的泽尔韦格综合征，它编码过氧化物酶蛋白Pex1p，属于AAA家族成员。带有表位标签的Pex1p在野生型中国仓鼠卵巢（CHO）细胞CHO-K1中表达。通过免疫荧光显微镜评估，Pex1p定位于细胞质中。用体外合成的35S标记的Pex6p（一种AAA家族过氧化物酶蛋白）与转染了HsPEX1的CHO-K1细胞裂解物一起孵育。使用抗Pex1p抗体对Pex1p进行免疫沉淀，结果同时回收了35S-Pex6p。相反，使用抗Pex6p抗体，从表达人Pex6p的CHO-K1细胞的免疫沉淀中获得了35S-Pex1p。这些结果有力地表明Pex1p和Pex6p相互作用。
