Castrillon PO, Arce A, Bonacho MG, Cardinali DP, Esquifino AI
Departamento de Bioquimica y Biologia Molecular III, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Biol Signals. 1998 Jan;7(1):25-32.
Male rats were grafted an anterior pituitary within breast muscles or received a sham operation on day 5 of life. At the 60th day of life, the sympathetic denervation of rat submaxillary lymph nodes was achieved by a bilateral sympathetic superior cervical ganglionectomy (SCGx; at 15.00 h). Rats were killed either 18 h later (acute SCGx) or after 12 days (chronic SCGx) to measure lipopolysaccharide (LPS)- and concanavalin A (ConA)-induced cell proliferation in submaxillary lymph nodes, submaxillary lymph node cellularity and serum prolactin levels. In control rats, acute SCGx significantly augmented LPS or ConA activity on lymph cells while chronic SCGx had no effect. In pituitary-grafted rats, acute SCGx depressed the mitogenic effect of LPS or ConA whereas chronic SCGx augmented it. A global inhibitory effect of surgical stress on submaxillary lymph node cellularity was found in rats subjected to SCGx or its sham operation 18 h earlier. Serum prolactin levels increased significantly in pituitary-grafted rats, particularly in those subjected to chronic SCGx. In pituitary-grafted rats, a significant effect of acute SCGx was apparent, with serum prolactin levels augmenting about twice in sham-SCGx rats, and to a significantly less extent in acute SCGx rats. The results provide further evidence of the immunomodulatory role of local sympathetic nerves in submaxillary lymph nodes.
雄性大鼠在出生后第5天于胸肌内移植垂体前叶或接受假手术。在出生后第60天，通过双侧交感神经颈上神经节切除术（SCGx；于15:00进行）实现大鼠颌下淋巴结的交感神经去支配。大鼠在18小时后（急性SCGx）或12天后（慢性SCGx）处死，以测量脂多糖（LPS）和伴刀豆球蛋白A（ConA）诱导的颌下淋巴结细胞增殖、颌下淋巴结细胞数量及血清催乳素水平。在对照大鼠中，急性SCGx显著增强LPS或ConA对淋巴细胞的活性，而慢性SCGx则无此作用。在垂体移植大鼠中，急性SCGx抑制LPS或ConA的促有丝分裂作用，而慢性SCGx则增强该作用。在18小时前接受SCGx或其假手术的大鼠中，发现手术应激对颌下淋巴结细胞数量具有整体抑制作用。垂体移植大鼠的血清催乳素水平显著升高，尤其是那些接受慢性SCGx的大鼠。在垂体移植大鼠中，急性SCGx的作用显著，假手术-SCGx大鼠的血清催乳素水平增加约两倍，而急性SCGx大鼠增加的程度明显较小。这些结果为局部交感神经在颌下淋巴结中的免疫调节作用提供了进一步的证据。
