Gatti R, Roveri P, Bonazzi D, Cavrini V
Dipartimento di Scienze Farmaceutiche, Università di Bologna, Italy.
J Pharm Biomed Anal. 1997 Nov;16(3):405-12. doi: 10.1016/s0731-7085(97)00078-2.
The use of 2-chloro-6,7-dimethoxy-3-quinolinecarboxaldehyde as a fluorogenic labelling reagent in pre-column derivatization for the HPLC separation of chlorophenols has been investigated. The compound reacts (50 min at 110 degrees C) with 2- and 4-chlorophenols to give fluorescent ethers that can be separated by reversed-phase HPLC and detected at lambda exc = 360 nm, lambda em = 500 nm. The experimental conditions for derivatization and chromatographic separation are discussed. Applications for the determination of chlorocresol (4-chloro-3-cresol) and chloroxylenol (4-chloro-3,5-xylenol) in pharmaceutical formulations (creams, ointments) are described.
研究了将2-氯-6,7-二甲氧基-3-喹啉甲醛用作柱前衍生化的荧光标记试剂，用于高效液相色谱法分离氯酚。该化合物与2-氯酚和4-氯酚反应（110℃下反应50分钟），生成可通过反相高效液相色谱分离并在激发波长λex = 360nm、发射波长λem = 500nm处检测的荧光醚。讨论了衍生化和色谱分离的实验条件。描述了该方法在药物制剂（乳膏、软膏）中氯甲酚（4-氯-3-甲酚）和氯二甲酚（4-氯-3,5-二甲酚）测定中的应用。
