Viñas P, Campillo N, López-Erroz C, Hernández-Córdoba M
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
J Pharm Biomed Anal. 1997 Nov;16(3):453-7. doi: 10.1016/s0731-7085(97)00074-5.
In the present study, new flow injection procedures for the determination of p-hydroxyphenylglycine using either photometric or fluorimetric detection are proposed. The methods are based on the reaction of the amino acid with o-phthalaldehyde and 2-mercaptoethanol. The calibration graphs based on peak area were linear in the ranges 20-300 ng ml-1 with fluorimetric detection and 5-60 micrograms ml-1 using the photometric mode. The detection limit calculated for the fluorimetric procedure was 10 ng ml-1. The method was applied to the determination of the free p-hydroxyphenylglycine present in industrial pharmaceutical samples of a Dane salt.
