López-Erroz C, Viñas P, Cerdán F J, Hernández-Córdoba M
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, E-30071 Murcia, Spain.
Talanta. 2000 Oct 2;53(1):47-53. doi: 10.1016/s0039-9140(00)00381-7.
A new procedure for the determination of clenbuterol is proposed using flow-injection and fluorimetric detection. The method is based on the derivatization reaction of the primary amine group with o-phthalaldehyde in the presence of 2-mercaptoethanol. The calibration graph based on peak area was linear in the range 0.2-5 mug ml(-1) and the detection limit was 0.06 mug ml(-1). The method was validated using a reference spectrophotometric procedure and was applied to the determination of the drug in commercial pharmaceutical preparations.
提出了一种采用流动注射和荧光检测法测定克伦特罗的新方法。该方法基于伯胺基团在2-巯基乙醇存在下与邻苯二甲醛的衍生化反应。基于峰面积的校准曲线在0.2 - 5 μg ml⁻¹范围内呈线性，检测限为0.06 μg ml⁻¹。该方法通过参考分光光度法进行了验证，并应用于商业药物制剂中该药物的测定。
